Document Detail

Simultaneous determination of gemcitabine and gemcitabine-squalene by liquid chromatography-tandem mass spectrometry in human plasma.
MedLine Citation:
PMID:  17851141     Owner:  NLM     Status:  MEDLINE    
Gemcitabine-squalene is a new prodrug that self-organizes in water forming nanoassemblies. It exhibits better anti-cancer properties in vitro and in vivo than gemcitabine. A liquid chromatography/tandem mass spectrometry assay of gemcitabine-squalene and gemcitabine was developed in human plasma in order to quantitate gemcitabine and its squalene conjugate. After protein precipitation with acetonitrile/methanol (90/10, v/v), the compounds were analyzed by reversed-phase high performance liquid chromatography and detected by tandem mass spectrometry using multiple reaction monitoring. The method was linear over the concentration range of 10-10,000 ng/ml of human plasma for both compounds with an accuracy lower than 10.4% and a precision below 14.8%. The method showed a lower limit of quantitation of 10 ng/ml of human plasma for dFdC and dFdC-SQ. A preliminary in vivo study in mice was shown as application of the method as no significant difference between human and mice plasma for the analysis of dFdC and dFdC-SQ was demonstrated.
Hania Khoury; Alain Deroussent; L Harivardhan Reddy; Patrick Couvreur; Gilles Vassal; Angelo Paci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-22
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  858     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-09     Completed Date:  2007-12-19     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-8     Citation Subset:  IM    
UPRES EA3535 Pharmacology and New Cancer Treatments, Institute Gustave Roussy - IFR54 and Paris XI University, 94800 Villejuif, France.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Deoxycytidine / analogs & derivatives*,  blood,  chemistry
Molecular Structure
Reproducibility of Results
Squalene / blood*,  chemistry
Tandem Mass Spectrometry / methods*
Reg. No./Substance:
111-02-4/Squalene; 951-77-9/Deoxycytidine; B76N6SBZ8R/gemcitabine

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