| Simultaneous determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine in Kampo medicines by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 10704044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simultaneous high-performance liquid chromatographic method for the determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine (ephedrine alkaloids) in Kampo medicines which contain Ephedrae Herba was established. The analysis can be accomplished within 25 min with a Wakosil-II 5C18 HG column by isocratic elution using a mixture of water, acetonitrile and sodium dodecyl sulfate (65:35:0.4) as the mobile phase at a flow-rate of 1.0 ml min(-1), and detection at 210 nm. The detection limits of ephedrine alkaloids are 0.37-1.06 microM per injection (5 microl). This method was applied to analyze the quantities in eight Kampo decoctions; Mao-to, Makyo-yokukan-to, Makyo-kanseki-to, Yokuinin-to, Sho-seiryu-to, Keima-kakuhan-to, Kakkon-to and Kakkon-to-ka-senkyu-sin'i. The concentration (per Ephedrae Herba gram) of ephedrine alkaloids was higher in the Makyo-kanseki-to decoction than in the others. Calcium sulfate from Gypsum Fibrosum raised ephedrine alkaloids dissolution in the Makyo-kanseki-to decoction. |
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Authors:
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N Okamura; H Miki; T Harada; S Yamashita; Y Masaoka; Y Nakamoto; M Tsuguma; H Yoshitomi; A Yagi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 20 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1999 Jun |
Date Detail:
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Created Date: 2000-04-04 Completed Date: 2000-04-04 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 363-72 Citation Subset: IM |
Affiliation:
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Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Central Nervous System Stimulants
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analysis* Chromatography, High Pressure Liquid Drugs, Chinese Herbal / analysis* Ephedrine / analogs & derivatives*, analysis* Medicine, Kampo Phenylpropanolamine / analysis Reference Standards Solutions Solvents |
| Chemical | |
Reg. No./Substance:
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0/Central Nervous System Stimulants; 0/Drugs, Chinese Herbal; 0/Solutions; 0/Solvents; 14838-15-4/Phenylpropanolamine; 17605-71-9/N-methylephedrine; 299-42-3/Ephedrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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