Document Detail

Simultaneous determination of delta G, delta H and delta S by an automatic microcalorimetric titration technique. Application to protein ligand binding.
MedLine Citation:
PMID:  7142659     Owner:  NLM     Status:  MEDLINE    
A methodological study has been made with a syringe titration unit attached to an LKB batch microcalorimeter. The precision and accuracy of the instrument assembly have been evaluated by neutralization reactions and by dilution of sucrose solutions. As an example, heat quantities on the order of 10 mJ accompanying the addition of 10 microliter titrant solution could be determined with an accuracy of better than 1%. A stepwise titration procedure was used to characterize the binding of indole-3-propionic acid to alpha-chymotrypsin. The following thermodynamic data were obtained (25 degrees C, acetate buffer, pH 5.80): delta G0=-18.46 +/- 0.17 kJ X mol-1, delta H0=-15.26 +/- 0.20 kJ X mol-1, delta S0 = 10.85 +/- 1.21 JK-1 X mol-1.
A Chen; I Wadsö
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  6     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1983-01-07     Completed Date:  1983-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  307-16     Citation Subset:  IM    
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MeSH Terms
Calorimetry / methods*
Chymotrypsin / metabolism*
Indoles / metabolism*
Reg. No./Substance:
0/Indoles; 830-96-6/indolepropionic acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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