Document Detail

Simultaneous determination of cysteine, cystine and 18 other amino acids in various matrices by high-performance liquid chromatography.
MedLine Citation:
PMID:  10081828     Owner:  NLM     Status:  MEDLINE    
An extraction protocol and a reversed-phase high-performance liquid chromatographic method for the simultaneous determination of cysteine, cystine and 18 other amino acids in biological and vegetable samples are described. Among the different methods proposed for amino acid determination, phenylisothiocyanate was used as the reagent for derivatization. Chromatograms obtained in the analysis of standard solutions and actual samples are reported, together with regression equation, correlation coefficient (> 0.999 for all), limit of detection and recoveries (between 86 and 102% for all the examined matrices) for each amino acid. Practical protocol and method applications in normal patients and patients affected by different pathologies, and in algal products are discussed.
L Campanella; G Crescentini; P Avino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  833     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-29     Completed Date:  1999-03-29     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
Department of Chemistry, University of Rome La Sapienza, Italy.
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MeSH Terms
Amino Acids / analysis*,  blood,  urine
Chromatography, High Pressure Liquid / methods*
Cysteine / analysis*,  blood,  urine
Cystine / analysis*,  blood
Cystinuria / urine
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Thyroid Gland / chemistry
Vegetables / chemistry
Reg. No./Substance:
0/Amino Acids; 52-90-4/Cysteine; 56-89-3/Cystine

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