Document Detail


Simultaneous determination of cysteine, cystine and 18 other amino acids in various matrices by high-performance liquid chromatography.
MedLine Citation:
PMID:  10081828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An extraction protocol and a reversed-phase high-performance liquid chromatographic method for the simultaneous determination of cysteine, cystine and 18 other amino acids in biological and vegetable samples are described. Among the different methods proposed for amino acid determination, phenylisothiocyanate was used as the reagent for derivatization. Chromatograms obtained in the analysis of standard solutions and actual samples are reported, together with regression equation, correlation coefficient (> 0.999 for all), limit of detection and recoveries (between 86 and 102% for all the examined matrices) for each amino acid. Practical protocol and method applications in normal patients and patients affected by different pathologies, and in algal products are discussed.
Authors:
L Campanella; G Crescentini; P Avino
Related Documents :
19588888 - Fluorous tbaf: a convenient and selective reagent for fluoride-mediated deprotections.
6527238 - Determination of benzalkonium chloride by gas chromatography.
18986668 - Determination of aromatic amines in water samples by capillary electrophoresis with amp...
22540938 - Rapid isolation and identification of active anti-oxidant ingredients from gongju using...
9847008 - Occurrence of cocaine in urine of substance-abuse treatment patients.
19714968 - Lc determination of albiflorin and paeoniflorin in bai shao (paeonia lactiflora) as a r...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  833     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-29     Completed Date:  1999-03-29     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Rome La Sapienza, Italy. campanellal@axrma.uniromal.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*,  blood,  urine
Chromatography, High Pressure Liquid / methods*
Cysteine / analysis*,  blood,  urine
Cystine / analysis*,  blood
Cystinuria / urine
Humans
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Thyroid Gland / chemistry
Vegetables / chemistry
Chemical
Reg. No./Substance:
0/Amino Acids; 52-90-4/Cysteine; 56-89-3/Cystine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A high-sensitive spectrofluorimetric method for the determination of micromolar concentrations of ir...
Next Document:  Enrichment of low-copy-number gene products by hydrophobic interaction chromatography.