Document Detail


Simultaneous determination of creatinine and pseudouridine concentrations in bovine plasma by reversed-phase liquid chromatography with photodiode array detection.
MedLine Citation:
PMID:  12505775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, rapid method for the simultaneous determination of creatinine and pseudouridine in bovine plasma is described. Plasma was de-proteinised, concentrated, and chromatographed for 15 min on a C(18) column. Analytes were detected at an optimum wavelength (262 nm) and the internal standard (cimetidine) was detected at 220 nm. The pH of analysis was between 6.5 and 7 where both analytes exist in single chemical forms giving maximum accuracy. Recoveries of both analytes were above 96%. Lowest detectable amounts of creatinine and pseudouridine were 0.28 nmol and 9.0 pmol, and the typical levels detected (+/-SD) were 60 (+/-2.8) and 2.3 (+/-0.10) micromol/L, respectively.
Authors:
A K Hewavitharana; H L Bruce
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  784     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2002-12-30     Completed Date:  2003-07-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  275-81     Citation Subset:  IM    
Affiliation:
Cooperative Research Centre for Cattle and Beef Quality, CSIRO, Food Science Australia, 4170, Cannon Hill, Queensland, Australia. a.hewavitharana@foodscience.afisc.csiro.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography, Liquid / methods*
Creatinine / blood*
Pseudouridine / blood*
Reference Standards
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / methods*
Chemical
Reg. No./Substance:
1445-07-4/Pseudouridine; 60-27-5/Creatinine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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