Document Detail


Simultaneous determination of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) in foods by selected reaction monitoring.
MedLine Citation:
PMID:  19485211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) were determined simultaneously by selected reaction monitoring (SRM) mass spectrometry for different food matrixes. A small amount of starting sample was saponified and extracted before injection into a linear ion trap mass spectrometer equipped with an atmospheric pressure chemical ionization source. Dihydrotachysterol, which is absent from food and has a structure similar to that of vitamins D3 and D2, was used as an internal standard. Calibration curves for the 2 vitamins showed linearity with R2 values of 0.9999 and 0.9989 for vitamins D3 and D2, respectively. Limits of detection for vitamins D3 and D2 were 0.5 ng/g (1.3 pmol/g) and 1.75 ng/g (4.4 pmol/g) and limits of quantitation were 1.25 ng/g (3.24 pmol/g), and 3.75 ng/g (9.45 pmol/g), respectively. Accuracy and precision of the method were tested with the infant formula reference standard of the National Institute of Standards and Technology, which showed a relative standard deviation of 6%. Recoveries ranged from 95 to 105%. Several food products were tested with AOAC Method 982.29, which is currently in use for vitamins D3 and D2, and results were comparable within 6%.
Authors:
Gianluca Dimartino
Publication Detail:
Type:  Evaluation Studies; Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  511-7     Citation Subset:  IM    
Affiliation:
Kraft Foods, Physical and Chemical Characterization, 200 Deforest Ave, East Hanover, NJ 07936, USA. gianluca.dimartino@kraft.com
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MeSH Terms
Descriptor/Qualifier:
Cholecalciferol / analysis*,  standards
Chromatography, Liquid
Dietary Supplements / analysis,  standards
Ergocalciferols / analysis*,  standards
Food Analysis / methods*,  standards,  statistics & numerical data
Mass Spectrometry / methods*,  standards,  statistics & numerical data
Reference Standards
Chemical
Reg. No./Substance:
0/Ergocalciferols; 67-97-0/Cholecalciferol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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