| Simultaneous determination of all-trans-, 13-cis- and 9-cis-retinoic acid, their 4-oxo metabolites and all-trans-retinol in human plasma by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 8953164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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All-trans-retinoic acid (all-trans-RA) and 13-cis-retinoic acid (13-cis-RA), due to their effects on cell differentiation, proliferation and angiogenesis, improved treatment results in some malignancies. Pharmacokinetic studies of all-trans-RA and 13-cis-RA along with monitoring of retinoic acid metabolites may help to optimize retinoic acid therapy and to develop new effective strategies for the use of retinoic acids in cancer treatment. Therefore, we developed a HPLC method for the simultaneous determination in human plasma of the physiologically important retinoic acid isomers, all-trans-, 13-cis- and 9-cis-retinoic acid, their 4-oxo metabolites, 13-cis-4-oxoretinoic acid (13-cis-4-oxo-RA) and all-trans-4-oxoretinoic acid (all-trans-4-oxo-RA), and Vitamin A (all-trans-retinol). Analysis was performed on a silica gel column with UV detection at 350 nm using a binary multistep gradient composed of n-hexane, 2-propanol and glacial acetic acid. For liquid-liquid extraction a mixture of n-hexane, dichloromethane and 2-propanol was used. The limits of detection were 0.5 ng/ml for retinoic acids and 10 ng/ml for all-trans-retinol. The method showed good reproducibility for all components (within-day C.V.: 3.02-11.70%; day-to-day C.V.: 0.01-11.34%). Furthermore, 9-cis-4-oxoretinoic acid (9-cis-4-oxo-RA) is separated from all-trans-4-oxo-RA and 13-cis-4-oxo-RA. In case of clinical use of 9-cis-retinoic acid (9-cis-RA) the pharmacokinetics and metabolism of this retinoic acid isomer can also be examined. |
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Authors:
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C Lanvers; G Hempel; G Blaschke; J Boos |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 685 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1997-03-25 Completed Date: 1997-03-25 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 233-40 Citation Subset: IM |
Affiliation:
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Department of Pediatric Hematology and Oncology, University of Münster, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods* Circadian Rhythm Humans Reproducibility of Results Retinoids / blood*, chemistry*, metabolism Spectrophotometry, Ultraviolet Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Retinoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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