Document Detail


Simultaneous determination of L-glutamate, acetylcholine and dopamine in rat brain by a flow-injection biosensor system with microdialysis sampling.
MedLine Citation:
PMID:  18997377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A flow-injection biosensor system with an on-line microdialysis sampling system is proposed for the simultaneous detection of neurotransmitters (L-glutamate, acetylcholine and dopamine) released from rat brain cells. The dialysate collected in the sample loop from the microdialysis probe was automatically injected into the flow-injection line with a triple electrode arranged perpendicular to the flow direction. The triple electrode was constructed by hybridizing a poly(1,2-diaminobenzene) film to two enzyme sensing-parts which respond to L-glutamate and acetylcholine, and by coating a Nafion film on a remaining sensing part which responds to dopamine, respectively, without any cross-reactivity. The three sensing parts of the triple electrode responded linearly to the concentrations of L-glutamate and acetylcholine in the range of 0.002-5 mM and to that of dopamine in the range of 0.002-20 mM, respectively, without any interference from oxidizable species present in the dialysate. The proposed flow-injection analytical method could be applied to an in vivo assay of these neurotransmitters released from rat-brain cells by the continuous KCl stimulation.
Authors:
Toshio Yao; Gen Okano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  24     ISSN:  1348-2246     ISO Abbreviation:  Anal Sci     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-10     Completed Date:  2008-12-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1469-73     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Naka, Sakai, Osaka 599-8531, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / analysis
Animals
Biosensing Techniques / methods*
Brain / cytology
Brain Chemistry*
Dopamine / analysis
Electrodes
Enzymes, Immobilized
Flow Injection Analysis
Glutamic Acid / analysis
Neurons / metabolism
Neurotransmitter Agents / analysis*
Potassium Chloride / pharmacology
Rats
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Neurotransmitter Agents; 51-84-3/Acetylcholine; 56-86-0/Glutamic Acid; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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