| Simultaneous determination of DL: -lactic acid and DL: -3-hydroxybutyric acid enantiomers in saliva of diabetes mellitus patients by high-throughput LC-ESI-MS/MS. | |
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MedLine Citation:
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PMID: 22895741 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A simultaneous determination method for the enantiomers of chiral carboxylic acids by the combination of ultraperformance liquid chromatography and mass spectrometry (UPLC-MS/MS) has been developed. (S)(+)-1-(2-Pyrrolidinylmethyl)-pyrrolidine (S-PMP) was used as the derivatization reagent for the high-throughput determination of biological chiral carboxylic acids, i.e., lactic acid (LA) and 3-hydroxybutyric acid (HA). The S-PMP efficiently reacted with the carboxylic acids under mild conditions at room temperature in the presence of 2,2'-dipyridyl disulfide and triphenylphosphine. The resulting S-PMP derivatives were highly responsive in the electrospray ionization (ESI)-MS operating in the positive-ion mode and gave characteristic product ions during the MS/MS, which enabled the sensitive detection using selected reaction monitoring. The derivatization was effective for the enantiomeric separation of the chiral carboxylic acids, and the resolution values of DL: -LA and DL: -HA were 4.91 and 9.37, respectively. Furthermore, a rapid separation of the derivatives of DL: -LA and DL: -HA within 7 min was performed using the UPLC system. The limits of detection on the column were in the low femtogram range (5-12 fg). The proposed procedure was successfully applied for the determination of the D: - and L: -isomers of LA and HA in the saliva of diabetes mellitus (DM) patients and healthy volunteers. The D: -LA in DM patients was clearly higher than that in normal subjects. The derivatization followed by UPLC-ESI-MS/MS enabled the enantiomeric separation and detection of trace amounts of LA and HA in human saliva with a simple pretreatment and small sample volume. |
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Authors:
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Haruhito Tsutsui; Toshiki Mochizuki; Toshio Maeda; Ichiro Noge; Yutaka Kitagawa; Jun Zhe Min; Kenichiro Todoroki; Koichi Inoue; Toshimasa Toyo'oka |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-16 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: - ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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