Document Detail


Simultaneous determination of 16 purine derivatives in urinary calculi by gradient reversed-phase high-performance liquid chromatography with UV detection.
MedLine Citation:
PMID:  15833286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reversed-phase high-performance liquid chromatography (HPLC) method with ultraviolet detection has been developed for the analysis of purines in urinary calculi. The method using gradient of methanol concentration and pH was able to separate 16 compounds: uric acid, 2,8-dihydroxyadenine, xanthine, hypoxanthine, allopurinol and oxypurinol as well as 10 methyl derivatives of uric acid or xanthine (1-, 3-, 7- and 9-methyluric acid, 1,3-, 1,7- and 3,7-dimethyluric acid, 1-, 3- and 7-methylxanthine). Limits of detection for individual compounds ranged from 0.006 to 0.035 mg purine/g of the stone weight and precision (CV%) was 0.5-2.4%. The method enabled us to detect in human uric acid stones admixtures of nine other purine derivatives: natural metabolites (hypoxanthine, xanthine, 2,8-dihydroxyadenine) and methylated purines (1-, 3- and 7-methyluric acid, 1,3-dimethyluric acid, 3- and 7-methylxanthine) originating from the metabolism of methylxanthines (caffeine, theophylline and theobromine). The method allows simultaneous quantitation of all known purine constituents of urinary stones, including methylated purines, and may be used as a reference one for diagnosing disorders of purine metabolism and research on the pathogenesis of urolithiasis.
Authors:
Krzysztof Safranow; Zygmunt Machoy
Related Documents :
20378966 - Effects of a fermented barley extract on subjects with slightly high serum uric acid or...
3808146 - Hypouricemia due to increased tubular urate secretion.
3765656 - Terpenoid biotransformation in mammals. iv biotransformation of (+)-longifolene, (-)-ca...
1756626 - Effect of tarnish on copper release.
2831366 - Synthesis and pharmacological characterization of a series of leukotriene analogues wit...
560206 - Stimulation by acidic phospholipids of protein-catalyzed phosphatidylcholine transfer.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  819     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-18     Completed Date:  2005-09-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  229-35     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland. chrissaf@mp.pl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid / methods*
Humans
Purines / analysis*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / methods*
Urinary Calculi / chemistry*
Chemical
Reg. No./Substance:
0/Purines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analysis of protein fragmentation inhibition by an MMP-inhibitor in an in vivo model of heart failur...
Next Document:  Determination of the cyanide metabolite 2-aminothiazoline-4-carboxylic acid in urine and plasma by g...