Document Detail


Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction.
MedLine Citation:
PMID:  19131259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A multiplex polymerase chain reaction (PCR) was designed for the simultaneous detection of four viruses involved in reproductive and respiratory failure in pigs: porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV), classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted fragment of the specific viruses. The sensitivity of the multiplex PCR using purified plasmid constructs containing the specific viral target fragments was 2.58x10(7), 2.64x10(5), 2.66x10(7) and 2.73x10(5) copies for PRRSV, PCV-2, CSFV and PPV, respectively. Using the multiplex PCR, co-infections with these four viruses were identified in 26/76 (34.2%) piglets born from sows with reproductive failure in China. This method is a rapid, sensitive and cost-effective diagnostic tool for the routine surveillance of viral infections in pigs.
Authors:
Yonghou Jiang; Hanwu Shang; Hui Xu; Liangjun Zhu; Weijie Chen; Lingyan Zhao; Li Fang
Related Documents :
8620189 - In vivo replication of african swine fever virus (malawi '83) in neutrophils.
2230259 - Pichinde virus infection in strain 13 guniea pigs reduces intestinal protein reflection...
15511539 - Use of automated real-time reverse transcription-polymerase chain reaction (rt-pcr) to ...
19453469 - Seroprevalence of h1n1, h3n2 and h1n2 influenza viruses in pigs in seven european count...
15831949 - More recent swine vesicular disease virus isolates retain binding to coxsackie-adenovir...
2832999 - Subclinical aujeszky's disease virus infection in a pig herd and the characterisation o...
809359 - Growth of measles virus in cultures of rat glioma cells.
10550669 - Antigenicity and pathogenicity characteristics of molecularly cloned chicken anaemia vi...
2476109 - Epitope mapping of the lassa virus nucleoprotein using monoclonal anti-nucleocapsid ant...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-07
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  183     ISSN:  1532-2971     ISO Abbreviation:  Vet. J.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-5     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 Elsevier Ltd. All rights reserved.
Affiliation:
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China. yonghoujiang@zstu.edu.cn
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Circoviridae Infections / diagnosis,  veterinary
Circovirus / genetics,  isolation & purification
Classical Swine Fever / diagnosis
Classical swine fever virus / genetics,  isolation & purification
DNA Primers / chemistry
DNA, Viral / analysis
Parvoviridae Infections / diagnosis,  veterinary
Parvovirus, Porcine / genetics,  isolation & purification
Polymerase Chain Reaction / veterinary*
Porcine Reproductive and Respiratory Syndrome / diagnosis
Porcine respiratory and reproductive syndrome virus / genetics,  isolation & purification
RNA, Viral / analysis
Sensitivity and Specificity
Sentinel Surveillance / veterinary
Swine
Swine Diseases / diagnosis*,  virology
Virus Diseases / diagnosis,  veterinary*
Viruses / genetics,  isolation & purification*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Post-event spontaneous intrusive recollections and strength of memory for emotional events in men an...
Next Document:  The effect of medial versus lateral rectus muscle surgery on distance-near incomitance.