Document Detail

Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction.
MedLine Citation:
PMID:  19131259     Owner:  NLM     Status:  MEDLINE    
A multiplex polymerase chain reaction (PCR) was designed for the simultaneous detection of four viruses involved in reproductive and respiratory failure in pigs: porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV), classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted fragment of the specific viruses. The sensitivity of the multiplex PCR using purified plasmid constructs containing the specific viral target fragments was 2.58x10(7), 2.64x10(5), 2.66x10(7) and 2.73x10(5) copies for PRRSV, PCV-2, CSFV and PPV, respectively. Using the multiplex PCR, co-infections with these four viruses were identified in 26/76 (34.2%) piglets born from sows with reproductive failure in China. This method is a rapid, sensitive and cost-effective diagnostic tool for the routine surveillance of viral infections in pigs.
Yonghou Jiang; Hanwu Shang; Hui Xu; Liangjun Zhu; Weijie Chen; Lingyan Zhao; Li Fang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-07
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  183     ISSN:  1532-2971     ISO Abbreviation:  Vet. J.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-5     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 Elsevier Ltd. All rights reserved.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
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MeSH Terms
Circoviridae Infections / diagnosis,  veterinary
Circovirus / genetics,  isolation & purification
Classical Swine Fever / diagnosis
Classical swine fever virus / genetics,  isolation & purification
DNA Primers / chemistry
DNA, Viral / analysis
Parvoviridae Infections / diagnosis,  veterinary
Parvovirus, Porcine / genetics,  isolation & purification
Polymerase Chain Reaction / veterinary*
Porcine Reproductive and Respiratory Syndrome / diagnosis
Porcine respiratory and reproductive syndrome virus / genetics,  isolation & purification
RNA, Viral / analysis
Sensitivity and Specificity
Sentinel Surveillance / veterinary
Swine Diseases / diagnosis*,  virology
Virus Diseases / diagnosis,  veterinary*
Viruses / genetics,  isolation & purification*
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral; 0/RNA, Viral

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