| Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. | |
| | |
MedLine Citation:
|
PMID: 16162525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The objective of this study was to develop a multiplex real-time polymerase chain reaction (PCR) method for simultaneous detection of Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis directly from milk. A genetic marker specific for Staph. aureus was used for primers and dual-labeled probe design. The target for Strep. agalactiae primers and dual-labeled probe was selected from the cfb gene encoding the Christie-Atkins-Munch-Petersen factor. The plasminogen activator gene was the target for primers and dual-labeled probe design for Strep. uberis. Quarter milk samples (n = 192) were analyzed by the multiplex real-time PCR assay and conventional microbiological methods. An additional 57 quarter milk samples were analyzed in a separate real-time PCR assay for Strep. agalactiae only. Using an overnight enrichment step, the real-time PCR technique correctly identified 96.4% of all quarter milk samples; 91.7% of Staph. aureus, 98.2% of Strep. agalactiae, and 100% of Strep. uberis. Results of conventional microbiological methods were used to determine the sensitivity and specificity of the multiplex real-time PCR procedure. The sensitivity of the procedure to correctly identify Staph. aureus, Strep. agalactiae, and Strep. uberis directly from milk was 95.5%, and the specificity was 99.6%. Results of this study indicate that the multiplex real-time PCR procedure has the potential to be a valuable diagnostic technique for simultaneous identification of Staph. aureus, Strep. agalactiae, and Strep. uberis directly from quarter milk samples. |
| | |
Authors:
|
B E Gillespie; S P Oliver |
Related Documents
:
|
16085835 - Distribution of cryptosporidium genotypes in storm event water samples from three water... 20104385 - Characterization of listeria monocytogenes isolates of food and human origins from braz... 10995115 - Vidas enzyme-linked immunoflourescent assay for detection of listeria in foods: collabo... 15644105 - Performance of a new chromogenic plating medium for the isolation of listeria monocytog... 12217265 - Couples' gender role preferences and management of family food preferences. 11289025 - Dietary fluoride intake of children aged 3-7 years in remote areas of thailand. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of dairy science Volume: 88 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2005 Oct |
Date Detail:
|
Created Date: 2005-09-15 Completed Date: 2006-03-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 3510-8 Citation Subset: IM |
Affiliation:
|
Food Safety Center of Excellence, Department of Animal Science, The University of Tennessee, Knoxville 37996, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle DNA, Bacterial / analysis Female Genetic Markers Mastitis, Bovine / microbiology* Milk / microbiology* Polymerase Chain Reaction / methods* Sensitivity and Specificity Staphylococcus aureus / genetics, isolation & purification* Streptococcus / genetics, isolation & purification* Streptococcus agalactiae / genetics, isolation & purification* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial; 0/Genetic Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of Staphylococcus aureus isolated from chronically infected dairy goats.
Next Document: Milk quality and automatic milking: fat globule size, natural creaming, and lipolysis.