Document Detail

Simultaneous detection of imprinted gene expression (p57(KIP2)) and molecular cytogenetics (FICTION) in the evaluation of molar pregnancies.
MedLine Citation:
PMID:  20626178     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To simultaneously evaluate the p57(KIP2) antibody expression and genotype of individual cells from paraffin sections of molar pregnancies. STUDY DESIGN: Paraffin sections from 10 typical and unusual molar pregnancies were evaluated with the FICTION technique (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms), using immunofluorescence staining for the p57(KIP2) antibody and enumeration fluorescence in situ hybridization (FISH) probes. The unusual cases included androgenetic/ biparental chimeric complete hydatidiform moles (CHM) and mosaic partial hydatidiform moles (PHM). The unusual molar conceptions provided insight into interpreting atypical p57(KIP2) staining patterns and identifying androgenetic cells. RESULTS: The androgenetic/biparental chimeric CHMs demonstrated a negative p57(KIP2) Staining pattern for the androgenetic cells and positive staining for the biparental cells. Concordantly, the FISH results showed delineation between the androgenetic cells and the biparental cells, indicating 2 distinct genotypes. Also, in the 2 cases of mosaic PHM, the partial loss of p57(KIP2) antibody staining was due to mosaic loss of chromosome 11, assumed to be the maternal copy. This provides a biological explanation as to how false interpretation could occur when evaluating p57(KIP2) immunostaining results. CONCLUSION: The FICTION technique is a valuable ancillary tool for simultaneously evaluating the genotype and p57(KIP2) expression in unusual molar pregnancies.
Lori Hoffner; W Tony Parks; Steven H Swerdlow; Jason C Carson; Urvashi Surti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of reproductive medicine     Volume:  55     ISSN:  0024-7758     ISO Abbreviation:  J Reprod Med     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-14     Completed Date:  2010-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173343     Medline TA:  J Reprod Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-28     Citation Subset:  IM    
Magee-Women's Research Institute, Pittsburgh, PA 15213, USA.
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MeSH Terms
Autoantibodies / analysis*,  metabolism
Chimera / genetics*
Cyclin-Dependent Kinase Inhibitor p57 / genetics*,  immunology
Gene Expression
Hydatidiform Mole / genetics*,  pathology
In Situ Hybridization, Fluorescence
Karyotyping / methods
Uterine Neoplasms / genetics*,  pathology
Reg. No./Substance:
0/Autoantibodies; 0/Cyclin-Dependent Kinase Inhibitor p57

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