| Simultaneous detection of imprinted gene expression (p57(KIP2)) and molecular cytogenetics (FICTION) in the evaluation of molar pregnancies. | |
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MedLine Citation:
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PMID: 20626178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To simultaneously evaluate the p57(KIP2) antibody expression and genotype of individual cells from paraffin sections of molar pregnancies. STUDY DESIGN: Paraffin sections from 10 typical and unusual molar pregnancies were evaluated with the FICTION technique (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms), using immunofluorescence staining for the p57(KIP2) antibody and enumeration fluorescence in situ hybridization (FISH) probes. The unusual cases included androgenetic/ biparental chimeric complete hydatidiform moles (CHM) and mosaic partial hydatidiform moles (PHM). The unusual molar conceptions provided insight into interpreting atypical p57(KIP2) staining patterns and identifying androgenetic cells. RESULTS: The androgenetic/biparental chimeric CHMs demonstrated a negative p57(KIP2) Staining pattern for the androgenetic cells and positive staining for the biparental cells. Concordantly, the FISH results showed delineation between the androgenetic cells and the biparental cells, indicating 2 distinct genotypes. Also, in the 2 cases of mosaic PHM, the partial loss of p57(KIP2) antibody staining was due to mosaic loss of chromosome 11, assumed to be the maternal copy. This provides a biological explanation as to how false interpretation could occur when evaluating p57(KIP2) immunostaining results. CONCLUSION: The FICTION technique is a valuable ancillary tool for simultaneously evaluating the genotype and p57(KIP2) expression in unusual molar pregnancies. |
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Authors:
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Lori Hoffner; W Tony Parks; Steven H Swerdlow; Jason C Carson; Urvashi Surti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of reproductive medicine Volume: 55 ISSN: 0024-7758 ISO Abbreviation: J Reprod Med Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-07-14 Completed Date: 2010-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0173343 Medline TA: J Reprod Med Country: United States |
Other Details:
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Languages: eng Pagination: 219-28 Citation Subset: IM |
Affiliation:
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Magee-Women's Research Institute, Pittsburgh, PA 15213, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Autoantibodies
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analysis*,
metabolism Chimera / genetics* Cyclin-Dependent Kinase Inhibitor p57 / genetics*, immunology Female Gene Expression Humans Hydatidiform Mole / genetics*, pathology In Situ Hybridization, Fluorescence Karyotyping / methods Mosaicism Pregnancy Uterine Neoplasms / genetics*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Cyclin-Dependent Kinase Inhibitor p57 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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