Document Detail

Simultaneous detection of beta-galactosidase activity and surface antigen expression in viable haematopoietic cells.
MedLine Citation:
PMID:  7851157     Owner:  NLM     Status:  MEDLINE    
The quantitation of intracellular beta-galactosidase activity has been described for viable cells. By using the fluorogenic substrate fluorescein-di-beta-D-galactopyranoside (FDG) in conjunction with flow cytometry, the proportion of positive cells as well as the level of expression can be determined. In this paper we describe beta-galactosidase expression in lymphoid and myeloid cells from transgenic mice that widely express beta-galactosidase from an inserted lacZ transgene. Both foetal and adult haematopoietic tissues are able to express beta-galactosidase. The intracellular fluorescence reflecting beta-galactosidase activity can be readily combined with fluorescently labelled antibodies against cell surface antigens. Thus, beta-galactosidase can be used as a marker in transplantation experiments to study the development of lymphoid and myeloid precursor cells.
C N Berger; S S Tan; K S Sturm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  17     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-14     Completed Date:  1995-03-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  216-23     Citation Subset:  IM    
Children's Medical Research Institute, Wentworthville, New South Wales, Australia.
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MeSH Terms
Antigens, Surface / analysis*,  blood
Biological Markers
Crosses, Genetic
Flow Cytometry
Hematopoietic Stem Cells / enzymology*,  immunology*
Liver / embryology,  enzymology
Mice, Transgenic
Thymus Gland / embryology,  enzymology
beta-Galactosidase / biosynthesis,  metabolism*
Reg. No./Substance:
0/Antigens, Surface; 0/Biological Markers; 0/Fluoresceins; 0/Galactosides; 17817-20-8/fluorescein-digalactoside; EC

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