Document Detail

Simultaneous demonstration of glia- and glioma-associated antigens in human astrocytomas.
MedLine Citation:
PMID:  3162445     Owner:  NLM     Status:  MEDLINE    
Glial fibrillary acidic protein (GFAP) and glioma-associated antigens (GAA) defined by monoclonal antibodies (MAbs) were demonstrated simultaneously in human astrocytoma tissue. GFAP was stained by PAP-method, GAA were visualized by avidin-biotin-technique using alkaline phosphatase. In primary and secondary tumors as well as in tissue culture heterogeneity of GFAP- and GAA-expression is obvious. GFAP is mostly restricted to cell processes and less marked in the perinuclear space. Depending on the individual antibody, MAbs-positive material is located either in the tumor cell plasma (MUC 8-22) or on cell surface membranes (MUC 2-63). There is remarkable expression of GAA in cell clusters which fail to express GFAP. At higher magnification, 3 types of cellular reactivity are detectable: (a) cells which react only with anti-GFAP, (b) cells which react only with anti-GAA and (c) cells which express both, GFAP and GAA, especially those of protoplasmic astrocyte type. These cells also occur in subcutaneous tumor grafts, and may thus represent not only a reactive event, but be part of tumor cell populations.
T Bilzer; B Martin; D Stavrou; E Keiditsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Supplement = Journal international du cancer. Supplement     Volume:  2     ISSN:  0898-6924     ISO Abbreviation:  Int. J. Cancer Suppl.     Publication Date:  1988  
Date Detail:
Created Date:  1988-05-02     Completed Date:  1988-05-02     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8710267     Medline TA:  Int J Cancer Suppl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  IM    
Department of Neuropathology, University of Düsseldorf, FRG.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Antigens, Neoplasm / analysis*
Astrocytoma / immunology*
Brain Neoplasms / immunology*
Glial Fibrillary Acidic Protein / analysis*,  immunology
Glioma / immunology*
Mice, Inbred BALB C
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Glial Fibrillary Acidic Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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