Document Detail


Simultaneous demonstration of glia- and glioma-associated antigens in human astrocytomas.
MedLine Citation:
PMID:  3162445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glial fibrillary acidic protein (GFAP) and glioma-associated antigens (GAA) defined by monoclonal antibodies (MAbs) were demonstrated simultaneously in human astrocytoma tissue. GFAP was stained by PAP-method, GAA were visualized by avidin-biotin-technique using alkaline phosphatase. In primary and secondary tumors as well as in tissue culture heterogeneity of GFAP- and GAA-expression is obvious. GFAP is mostly restricted to cell processes and less marked in the perinuclear space. Depending on the individual antibody, MAbs-positive material is located either in the tumor cell plasma (MUC 8-22) or on cell surface membranes (MUC 2-63). There is remarkable expression of GAA in cell clusters which fail to express GFAP. At higher magnification, 3 types of cellular reactivity are detectable: (a) cells which react only with anti-GFAP, (b) cells which react only with anti-GAA and (c) cells which express both, GFAP and GAA, especially those of protoplasmic astrocyte type. These cells also occur in subcutaneous tumor grafts, and may thus represent not only a reactive event, but be part of tumor cell populations.
Authors:
T Bilzer; B Martin; D Stavrou; E Keiditsch
Related Documents :
19337695 - Delivery of temozolomide to the tumor bed via biodegradable gel matrices in a novel mod...
17547775 - O6-methylguanine-dna methyltransferase is downregulated in transformed astrocyte cells:...
14531575 - Invasion as limitation to anti-angiogenic glioma therapy.
12949415 - An anaplastic astrocytoma (optic chiasmatic-hypothalamic glioma) in a dog.
23219755 - Combined stimulation of tlr9 and 4.1bb augments trp2 peptide vaccine-mediated melanoma ...
22842665 - Colorectal lateral spreading tumor subtypes: clinicopathology and outcome of endoscopic...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Supplement = Journal international du cancer. Supplement     Volume:  2     ISSN:  0898-6924     ISO Abbreviation:  Int. J. Cancer Suppl.     Publication Date:  1988  
Date Detail:
Created Date:  1988-05-02     Completed Date:  1988-05-02     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8710267     Medline TA:  Int J Cancer Suppl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  IM    
Affiliation:
Department of Neuropathology, University of Düsseldorf, FRG.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Antigens, Neoplasm / analysis*
Astrocytoma / immunology*
Brain Neoplasms / immunology*
Glial Fibrillary Acidic Protein / analysis*,  immunology
Glioma / immunology*
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Glial Fibrillary Acidic Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Elevated serum HMFG antigen levels in breast and ovarian cancer patients measured with a sandwich EL...
Next Document:  Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft...