Document Detail


Simultaneous collection of topographic and fluorescent images of barley chromosomes by scanning near-field optical/atomic force microscopy.
MedLine Citation:
PMID:  12113628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study investigated the applicability of scanning near-field optical/atomic force microscopy (SNOM/AFM) to observations of YOYO-1 stained barley chromosomes. Using this technique, topographic and fluorescent images in the same portion were obtained simultaneously, thus enabling the precise analysis of fluorescent structures in relation to the morphology of chromosomes. In YOYO-1 stained chromosomes, the fluorescent intensity roughly reflected the local amount and/or density of DNA in chromosomes. R-banding of chromosomes stained with YOYO-1 and methyl green was also observed clearly as bright spots of various sizes by this microscope. Thus, the SNOM/AFM is expected to become a useful tool for analysing the structure and function of chromosomes more precisely than before.
Authors:
Tomoyuki Yoshino; Shigeru Sugiyama; Shoji Hagiwara; Tatsuo Ushiki; Toshio Ohtani
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Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of electron microscopy     Volume:  51     ISSN:  0022-0744     ISO Abbreviation:  J Electron Microsc (Tokyo)     Publication Date:  2002  
Date Detail:
Created Date:  2002-07-12     Completed Date:  2002-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7611157     Medline TA:  J Electron Microsc (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  199-203     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Benzoxazoles
Chromosome Banding / methods
Chromosomes / ultrastructure*
DNA, Plant / analysis
Fluorescent Dyes
Hordeum / genetics*
Intercalating Agents
Methyl Green
Microscopy, Atomic Force / instrumentation,  methods*
Microscopy, Fluorescence / methods
Quinolinium Compounds
Chemical
Reg. No./Substance:
0/Benzoxazoles; 0/DNA, Plant; 0/Fluorescent Dyes; 0/Intercalating Agents; 0/Quinolinium Compounds; 143413-85-8/1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; 82-94-0/Methyl Green

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