Document Detail


Simultaneous changes in the mechanical properties, quantitative collagen organization, and proteoglycan concentration of articular cartilage following canine meniscectomy.
MedLine Citation:
PMID:  10937624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanical properties and microstructure of articular cartilage from the canine tibial plateau were studied 12 weeks after total medial meniscectomy. The organization of the birefringent collagen network was measured with quantitative polarized light microscopy to determine the thickness and the degree of organization of the superficial and deep zones. The zonal concentration of sulfated glycosaminoglycan was quantified with digital densitometry of safranin-O staining. Equilibrium compressive and shear properties, as well as dynamic shear properties, were measured at sites adjacent to those of microstructural analysis. The results evinced significant loss of cartilage function following meniscectomy, with decreases of 20-50% in the compressive and shear moduli. There was no evidence of alterations in the degree of collagen fibrillar organization, although a complete loss of the surface zone was seen in 60% of the samples that underwent meniscectomy. Meniscectomy resulted in a decreased concentration of sulfated glycosaminoglycan, and significant positive correlations were found between the equilibrium compressive modulus and the glycosaminoglycan content. Furthermore, the shear properties of cartilage correlated directly with collagen fibrillar organization measured at the superficial zone of corresponding sites. These findings demonstrate that meniscectomy leads to impaired mechanical function of articular cartilage, with significant evidence of quantitative correlations between cartilage microstructure and mechanics.
Authors:
M A LeRoux; J Arokoski; T P Vail; F Guilak; M M Hyttinen; I Kiviranta; L A Setton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  18     ISSN:  0736-0266     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-17     Completed Date:  2000-08-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  383-92     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Duke University Medical Center, Duke University, Durham, North Carolina 27708-0281, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Cartilage, Articular / chemistry*,  physiology
Collagen / analysis*
Dogs
Glycosaminoglycans / analysis
Menisci, Tibial / surgery*
Osteoarthritis / etiology
Proteoglycans / analysis*
Grant Support
ID/Acronym/Agency:
AR45092/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Proteoglycans; 0/glucosaminoglycans; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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