| Simultaneous cell surface phenotype and cell cycle analysis of lymphocytes by flow cytometry. | |
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MedLine Citation:
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PMID: 6205045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for the simultaneous measurement of cell surface components and nucleic acids (DNA and RNA) of human lymphocytes by flow cytometry has been developed, thereby providing a means of analyzing cell surface changes during the various phases of the cell cycle. Unfixed cells were coated with fluorescein-conjugated concanavalin A (F Con A) or surface antigen-specific antibody, fixed sequentially with paraformaldehyde and methanol, treated with specific nucleases, and then stained with propidium iodide. Neither portion of the procedure (cell surface staining, nucleic acid staining) interfered significantly with the other. Cell cycle phases of phytohemagglutinin-stimulated human lymphocytes as determined by this method were comparable with those identified by acridine orange staining. Cell cycle-specific blocking agents were used to additionally demonstrate the specificity of the staining procedure. Simultaneous measurement of cell cycle phase and detection of surface receptors for Con A and T lymphocyte surface determinants was performed with this method. |
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Authors:
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A Noronha; D P Richman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 32 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1984 Aug |
Date Detail:
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Created Date: 1984-08-30 Completed Date: 1984-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 821-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, Surface / analysis Cell Cycle DNA / blood Flow Cytometry Humans Lymphocyte Activation Lymphocytes / cytology* Phenotype RNA / blood Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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