Document Detail

Simultaneous cell by cell study of both DNA fragmentation and chromosomal segregation in spermatozoa from chromosomal rearrangement carriers.
MedLine Citation:
PMID:  23288665     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Balanced chromosomal translocations are found in one out of 500 subjects in the general population. They usually do not carry any phenotypic consequences, except for possible infertility and for the production of unbalanced gametes leading to spontaneous abortions or chromosomal syndromes in the offspring. An association between chromosomal rearrangements and increased apoptosis markers has been demonstrated on a global scale in sperm samples of translocation and inversion carriers. In order to specify which kind of sperm cells is subject to an increased apoptosis process, this present study was aimed to analyse both chromosomal segregation and DNA fragmentation, sperm cell by sperm cell.
METHODS: Six patients carrying a chromosomal rearrangement (three reciprocal translocations, two Robertsonian translocations, and one chromosomal pericentric inversion) were included in a retrospective manner. Both DNA fragmentation and chromosomal segregation in spermatozoa were evaluated simultaneously using a modified TUNEL assay associated with FISH. Two thousand spermatozoa were analysed for each patient.
RESULTS: We showed a higher proportion of spermatozoa with fragmented DNA among the unbalanced sperm cells, compared to the balanced ones, in all six patients.
CONCLUSIONS: These results suggest an increased fragility of unbalanced spermatozoa to exogenous fragmentation factors. The exact mechanisms of those processes remain to be elucidated.
Alexandre Rouen; Ketty Pyram; Xavier Pollet-Villard; Capucine Hyon; Maud Dorna; Sandrine Marques; Sandra Chantot-Bastaraud; Nicole Joyé; Nino Guy Cassuto; Jean-Pierre Siffroi
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Publication Detail:
Type:  Journal Article     Date:  2013-01-04
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-09-23     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  383-90     Citation Subset:  IM    
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MeSH Terms
Apoptosis / genetics
Chromosome Segregation / genetics*
DNA Fragmentation*
In Situ Hybridization, Fluorescence
Meiosis / genetics
Spermatozoa / cytology*
Translocation, Genetic / genetics*

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