Document Detail


Simultaneous analysis of gamma-hydroxybutyric acid, gamma-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography.
MedLine Citation:
PMID:  11909651     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of gamma-hydroxybutyric acid (GHB), gamma-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0mM sodium barbital and 150.0mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients (r(2)) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1mg/l, 0.34 and 0.25g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. (n = 9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions.
Authors:
Johan Dahlén; Thyrsa Vriesman
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  125     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-22     Completed Date:  2002-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  113-9     Citation Subset:  IM    
Affiliation:
SKL-National Laboratory of Forensic Science, SE-581 94, Linköping, Sweden. drog@skl.police.se
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MeSH Terms
Descriptor/Qualifier:
4-Butyrolactone / analysis*
Butylene Glycols / analysis*
Chromatography, Micellar Electrokinetic Capillary / methods*
Hydroxybutyrates / analysis*
Solvents / analysis*
Chemical
Reg. No./Substance:
0/Butylene Glycols; 0/Hydroxybutyrates; 0/Solvents; 110-63-4/1,4-butanediol; 591-81-1/4-hydroxybutyric acid; 96-48-0/4-Butyrolactone

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