Document Detail


Simultaneous quantification of erlotinib, gefitinib, and imatinib in human plasma by liquid chromatography tandem mass spectrometry.
MedLine Citation:
PMID:  19865001     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A quantitative liquid chromatography (LC)-mass spectrometry (MS)/MS method in human plasma was developed and validated for the tyrosine kinase inhibitors erlotinib, gefitinib, and imatinib in human plasma. Pre-treatment of the samples was achieved by using liquid-liquid extraction using D-8 imatinib as internal standard. Separation was performed on a Waters Alliance 2795 LC system using an XBridge RP18 column. The mass spectrometer Micromass was equipped with an electro spray ionization probe, operating in the positive mode. The calibration curves in plasma were linear for erlotinib, gefitinib, and imatinib over the concentration range of 5 to 3,000; 5 to 3,000, and 5 to 5,000 ng/mL, respectively. The intraday and interday accuracy ranged from 90% to 110% and the intraday and interday precision of the method was within 5%. The reported method provided the necessary linearity, precision, and accuracy to determine tyrosine kinase inhibitors in clinical research and for therapeutic drug monitoring.
Authors:
A Chahbouni; J C G den Burger; R M Vos; A Sinjewel; A J Wilhelm
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Therapeutic drug monitoring     Volume:  31     ISSN:  1536-3694     ISO Abbreviation:  Ther Drug Monit     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-03-19     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7909660     Medline TA:  Ther Drug Monit     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-7     Citation Subset:  IM    
Affiliation:
VU University Medical Center, Department of Clinical Pharmacology and Pharmacy, Amsterdam, The Netherlands. a.chahbouni@vumc.nl
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MeSH Terms
Descriptor/Qualifier:
Analytic Sample Preparation Methods
Calibration
Chromatography, High Pressure Liquid / methods*
Drug Monitoring / methods
Humans
Microchemistry
Piperazines / blood*
Pyrimidines / blood*
Quinazolines / blood*
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization / methods
Tandem Mass Spectrometry / methods*
Chemical
Reg. No./Substance:
0/Piperazines; 0/Pyrimidines; 0/Quinazolines; BKJ8M8G5HI/imatinib; J4T82NDH7E/erlotinib; S65743JHBS/gefitinib

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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