Document Detail


Simultaneous Quantification of Capsaicinoids and Ascorbic Acid from Pungent Peppers.
MedLine Citation:
PMID:  23081967     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The development of simultaneous extraction and determination of bioactive molecules from natural products is becoming more popular. The present study reports the development of a method for the simultaneous extraction and determination of both capsaicinoids and ascorbic acid in peppers. Capsaicin (341.61 µg/g), dihydrocapsaicin (119.91 µg/g) and ascorbic acid (2,109.60 µg/g) were extracted with 3% metaphosphoric acid-ethanol (2:8) as a solvent. The efficient extraction of capsaicinoids (412.61 µg/g) and ascorbic acid (2,785.93 µg/g) was achieved at a sample-to-solvent ratio of 1:8 after 30 minutes of sonication. Simultaneous separation of capsaicinoids and ascorbic acid was achieved using a Gemini C18 column with a gradient elution of 0.03M phosphoric acid and methanol. Capsaicinoids and ascorbic acid were simultaneously detected at 282 and 254 nm, respectively. The recovery of capsaicinoids ranged from 96.21 to 108.71%, and the recovery of ascorbic acid ranged from 97.01 to 98.83%. The limits of detection for capsaicin, dihydrocapsaicin and ascorbic acid were 0.24, 0.21 and 0.26 µg, respectively. Relative standard deviation for the intra-day and inter-day variability in the results was less than 3%, indicating that the method produced highly reproducible results. Therefore, this method enables the reproducible, simultaneous separation and quantification of capsaicinoids and ascorbic acid from peppers.
Authors:
Haejin Bae; Guddadarangavvanahally K Jayaprakasha; Kevin Crosby; John L Jifon; Bhimanagouda S Patil
Related Documents :
23411287 - The protein interactions and rheological properties of skim milk heated in the presence...
10584027 - Isolation of terrabacter sp. strain dde-1, which metabolizes 1, 1-dichloro-2,2-bis(4-ch...
24660447 - Differences in the chemical composition of arnica montana flowers from wild populations...
23949487 - Selective fluorescent detection of aspartic acid and glutamic acid employing dansyl hyd...
10763277 - Selective voltammetric and amperometric detection of uric acid with oxidized diamond fi...
1559937 - Dichloroacetate blocks endogenous opioid effects during inspiratory flow-resistive load...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-18
Journal Detail:
Title:  Journal of chromatographic science     Volume:  -     ISSN:  1945-239X     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77845.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Selectivity Tuning in Pressurized Capillary Electrochromatography with a Zwitterionic Monolithic Col...
Next Document:  RP-HPLC/Pre-Column Derivatization for Analysis of Omeprazole, Tinidazole, Doxycycline and Clarithrom...