| Simultaneous Quantification of Capsaicinoids and Ascorbic Acid from Pungent Peppers. | |
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MedLine Citation:
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PMID: 23081967 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The development of simultaneous extraction and determination of bioactive molecules from natural products is becoming more popular. The present study reports the development of a method for the simultaneous extraction and determination of both capsaicinoids and ascorbic acid in peppers. Capsaicin (341.61 µg/g), dihydrocapsaicin (119.91 µg/g) and ascorbic acid (2,109.60 µg/g) were extracted with 3% metaphosphoric acid-ethanol (2:8) as a solvent. The efficient extraction of capsaicinoids (412.61 µg/g) and ascorbic acid (2,785.93 µg/g) was achieved at a sample-to-solvent ratio of 1:8 after 30 minutes of sonication. Simultaneous separation of capsaicinoids and ascorbic acid was achieved using a Gemini C18 column with a gradient elution of 0.03M phosphoric acid and methanol. Capsaicinoids and ascorbic acid were simultaneously detected at 282 and 254 nm, respectively. The recovery of capsaicinoids ranged from 96.21 to 108.71%, and the recovery of ascorbic acid ranged from 97.01 to 98.83%. The limits of detection for capsaicin, dihydrocapsaicin and ascorbic acid were 0.24, 0.21 and 0.26 µg, respectively. Relative standard deviation for the intra-day and inter-day variability in the results was less than 3%, indicating that the method produced highly reproducible results. Therefore, this method enables the reproducible, simultaneous separation and quantification of capsaicinoids and ascorbic acid from peppers. |
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Authors:
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Haejin Bae; Guddadarangavvanahally K Jayaprakasha; Kevin Crosby; John L Jifon; Bhimanagouda S Patil |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-18 |
Journal Detail:
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Title: Journal of chromatographic science Volume: - ISSN: 1945-239X ISO Abbreviation: J Chromatogr Sci Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77845. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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