| Simultaneous POMC gene transfer to hypothalamus and brainstem increases physical activity, lipolysis and reduces adult-onset obesity. | |
| | |
MedLine Citation:
|
PMID: 21366729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pro-opiomelanocortin (POMC) neurons are identified in two brain sites, the arcuate nucleus of the hypothalamus and nucleus of the solitary tract (NTS) in brainstem. Earlier pharmacological and POMC gene transfer studies demonstrate that melanocortin activation in either site alone improves insulin sensitivity and reduces obesity. The present study, for the first time, investigated the long-term efficacy of POMC gene transfer concurrently into both sites in the regulation of energy metabolism in aged F344xBN rats bearing adult-onset obesity. Pair feeding was included to reveal food-independent POMC impact on energy expenditure. We introduced adeno-associated virus encoding either POMC or green fluorescence protein to the two brain areas in 22-month-old rats, then recorded food intake and body weight, assessed oxygen consumption, serum leptin, insulin and glucose, tested voluntary wheel running, analysed POMC expression, and examined fat metabolism in brown and white adipose tissues. POMC mRNA was significantly increased in both the hypothalamus and NTS region at termination. Relative to pair feeding, POMC caused sustained weight reduction and additional fat loss, lowered fasting insulin and glucose, and augmented white fat hormone-sensitive lipase activity and brown fat uncoupling protein 1 level. By wheel running assessment, the POMC animals ran twice the distance as the Control or pair-fed rats. Thus, the dual-site POMC treatment ameliorated adult-onset obesity effectively, involving a moderate hypophagia lasting ∼60 days, enhanced lipolysis and thermogenesis, and increased physical activity in the form of voluntary wheel running. The latter finding provides a clue for countering age-related decline in physical activity. |
| | |
Authors:
|
Yi Zhang; Enda Rodrigues; Gang Li; Yongxin Gao; Michael King; Christy S Carter; Nihal Tumer; Kit-Yan Cheng; Philip J Scarpace |
Related Documents
:
|
23419999 - Effects of modified foodservice practices in military dining facilities on ad libitum n... 21367929 - Differential mirna expression in omental adipose tissue and in the circulation of obese... 278859 - Enhancement of dl-ethionine-induced liver carcinogenesis in rats fed a choline-devoid d... 3811909 - Morphogenesis of septa in hepatic fibrosis induced by choline deficiency in rats. corre... 115309 - Carnitine content of liquid formulas and special diets. 20068559 - L-carnitine supplementation to diet: a new tool in treatment of nonalcoholic steatohepa... 1527369 - Contribution of colostral fat to thermogenesis and glucose homeostasis in the newborn pig. 9465169 - Functional properties of jaw and tongue muscles in rats fed a liquid diet after being w... 8785189 - Alcohol and the regulation of energy balance: overnight effects on diet-induced thermog... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-03-02 |
Journal Detail:
|
Title: The European journal of neuroscience Volume: 33 ISSN: 1460-9568 ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-18 Completed Date: 2011-09-16 Revised Date: 2013-06-11 |
Medline Journal Info:
|
Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France |
Other Details:
|
Languages: eng Pagination: 1541-50 Citation Subset: IM |
Copyright Information:
|
European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. No claim to original US government works. |
Affiliation:
|
Malcom Randall Veterans Affairs Medical Center, Gainesville, FL 32608, USA. yizhang@ufl.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aging
/
physiology Animals Arcuate Nucleus / physiology* Blood Glucose / metabolism Body Weight Dependovirus / genetics, metabolism Eating Energy Metabolism Gene Transfer Techniques* Humans Insulin / blood Leptin / blood Lipolysis / physiology* Male Motor Activity / physiology* Obesity / physiopathology* Pro-Opiomelanocortin / genetics*, metabolism Rats Rats, Inbred F344 Solitary Nucleus / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
1 P30 AG028740/AG/NIA NIH HHS; AG026159/AG/NIA NIH HHS; AG20985/AG/NIA NIH HHS; R01 AG026159/AG/NIA NIH HHS; R01 AG026159-04/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 0/Insulin; 0/Leptin; 66796-54-1/Pro-Opiomelanocortin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immortalized cell lines for real-time analysis of circadian pacemaker and peripheral oscillator prop...
Next Document: Endogenous proBDNF is a negative regulator of migration of cerebellar granule cells in neonatal mice...