Document Detail

Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples.
MedLine Citation:
PMID:  17263449     Owner:  NLM     Status:  MEDLINE    
A method has been developed for the simultaneous analysis of biogenic amines, amino acids, and the ammonium ion in wine and beer. Aminoenones formed by the reaction of amino acids, biogenic amines, and the ammonium ion with the derivatization reagent diethyl ethoxymethylenemalonate are separated by HPLC. Reaction takes place in methanolic alkaline medium for 30 min in an ultrasonic bath. Further heating at 70 degrees C for 2 h produces complete degradation of excess derivatization reagent and byproducts. Comparison of the results of ammonium analysis and enzymatic analysis showed a good correlation (r = 0.953). The proposed analytical method has the following advantages: easy derivatization of wines and beers; quantification of 24 amino acids, nine biogenic amines, and the ammonium ion in a single injection; use of the photodiode array detector; complete degradation of excess derivatization reagent during sample preparation; and detection limits below 0.40 mg/L for amino acids and below 0.06 mg/L for biogenic amines.
Sergio Gómez-Alonso; Isidro Hermosín-Gutiérrez; Esteban García-Romero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  608-13     Citation Subset:  IM    
Instituto de la Vid y el Vino de Castilla-La Mancha, Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Spain.
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MeSH Terms
Amino Acids / analysis*
Beer / analysis*
Biogenic Amines / analysis*
Chromatography, High Pressure Liquid / methods*
Quaternary Ammonium Compounds / analysis*
Wine / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Biogenic Amines; 0/Malonates; 0/Quaternary Ammonium Compounds; 87-13-8/diethyl (ethoxymethylene)malonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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