| Simultaneous Determination of Six Bioactive Compounds in Evodiae Fructus by High-Performance Liquid Chromatography with Diode Array Detection. | |
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MedLine Citation:
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PMID: 23377650 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A simple and reliable high-performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and validated for the simultaneous determination of six bioactive components, rutaevine, limonin, evodiamine, rutaecarpine, N-formyldihydrorutaecarpine and dihydroevocarpine, in the traditional Chinese medicine Evodiae Fructus (Wuzhuyu in Chinese). HPLC separation was conducted on an Agilent Eclipse C18 column (4.6 × 150 mm, 5 µm) at 35°C with a mixture of mobile phase A [tetrahydrofuran-0.02% phosphoric acid (16 : 35)] and mobile phase B (acetonitrile) (gradient elution as follows: 0 min, 22% B; 23 min, 22% B; 24 min, 75% B) at a flow rate of 1 mL/min, and the DAD detection wavelength was set at 220 nm. A linear relationship within the range of investigated concentrations was observed for the six compounds, with correlation coefficients greater than 0.999. The average recovery yields of the six compounds ranged from 98.39 to 104.96%. The HPLC-DAD method was validated by its repeatability [relative standard deviation (RSD) < 2.0%] and intra-day and inter-day precision (RSD < 2.0%). The method was successfully applied to the simultaneous determination of the six previously mentioned components in Evodiae Fructus. It is the first report of a simultaneous qualitative and quantitative analysis for three classes of bioactive components in Wuzhuyu, including the indolequinazoline alkaloids, quinolone alkaloid and limonoids. Based on these results, it is suggested, for possible future revision of the Chinese Pharmacopoeia, that the total contents of evodiamine and rutaecarpine are not less than 0.15% and the total contents of rutaevine and limonin are not less than 0.50%. |
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Authors:
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Xiaolong Tang; Zhifang Huang; Yan Chen; Yunhua Liu; Yuhong Liu; Junning Zhao; Jinhai Yi |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-1 |
Journal Detail:
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Title: Journal of chromatographic science Volume: - ISSN: 1945-239X ISO Abbreviation: J Chromatogr Sci Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Sichuan Academy of Chinese Medicine Science, Chengdu 610041, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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