| Simultaneous Detection of T Lymphocyte-Related Antigens (CD4/CD8, CD57, TCRβ) with Nuclei by Fluorescence-Based Immunohistochemistry in Paraffin-Embedded Human Lymph Node, Liver Cancer and Stomach Cancer. | |
| | |
MedLine Citation:
|
PMID: 21791906 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Objective: To develop a method for immunohistochemical staining of three different T lymphocyte antigens (CD4 or CD8, CD57 and TCRβ) on the same tissue section and to determine whether tissues have been infiltrated with T lymphocytes expressing these markers. Study Design: Commercially available antibodies were tested for immunohistochemical usefulness in a dye-based conventional single-immunostaining method after antigen retrieval on paraffin-embedded human lymph nodes. We searched for the combination of antibodies that could detect T lymphocyte antigens on the same section without any cross-reactivity and that have fluorescent signals robust enough to overcome paraffin autofluorescence. Results: Application of the antigen retrieval technique and the Sudan black B quenching technique enabled staining of paraffin-embedded tissue sections with fluorescent-labeled secondary antibodies. The combination of primary and secondary antibodies that could simultaneously detect the T lymphocyte antigens CD4 or CD8, CD57 and TCRβ in histochemical analysis of a paraffin-embedded human lymph node section was established, and was successfully applied to a human tissue section infiltrated with T lymphocytes that express these markers. Conclusion: The antibodies listed here would be helpful for histopathologists who wish to investigate T lymphocytes in the paraffin-embedded sections that have accumulated in pathology labs throughout the world. |
| | |
Authors:
|
Takahiro Nakata; Eiji Takayama; Hirohito Magari; Jun Kato; Nobuo Kondo; Ichinose Masao |
Related Documents
:
|
2094276 - Immunological approaches for manipulation of animal growth, body composition and fecund... 16893686 - Antibody production: polyclonal-derived biotherapeutics. 22113616 - Structure of hiv-1 gp120 v1/v2 domain with broadly neutralizing antibody pg9. 2275476 - Role of monoclonal antibody therapy in the treatment of infectious disease. 8784796 - Colonic motor response to ige-mediated mast cell degranulation in the hooded-lister rat. 20062896 - Anti-double stranded antibody. association with titers and fluorescence patterns of ant... |
Publication Detail:
|
Type: Journal Article Date: 2011-07-22 |
Journal Detail:
|
Title: Acta cytologica Volume: 55 ISSN: 0001-5547 ISO Abbreviation: Acta Cytol. Publication Date: 2011 |
Date Detail:
|
Created Date: 2011-07-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370307 Medline TA: Acta Cytol Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 357-63 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
|
Department of Health Science, Ishikawa Prefectural Nursing University, Kahoku, Ishikawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Three-dimensional nuclear luminance analysis in well-differentiated adenocarcinoma of the lung.
Next Document: A comparison of dacron versus Flocked nylon swabs for anal cytology specimen collection.