Document Detail


Simultaneous delivery of chemotherapeutic and thermal-optical agents to cancer cells by a polymeric (PLGA) nanocarrier: an in vitro study.
MedLine Citation:
PMID:  20694526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To test the effectiveness of a dual-agent-loaded PLGA nanoparticulate drug delivery system containing doxorubicin (DOX) and indocyanine green (ICG) in a DOX-sensitive cell line and two resistant cell lines that have different resistance mechanisms.
METHODS: The DOX-sensitive MES-SA uterine sarcoma cell line was used as a negative control. The two resistant cell lines were uterine sarcoma MES-SA/Dx5, which overexpresses the multidrug resistance exporter P-glycoprotein, and ovarian carcinoma SKOV-3, which is less sensitive to doxorubicin due to a p53 gene mutation. The cellular uptake, subcellular localization and cytotoxicity of the two agents when delivered via nanoparticles (NPs) were compared to their free-form administration.
RESULTS: The cellular uptake and cytotoxicity of DOX delivered by NPs were comparable to the free form in MES-SA and SKOV-3, but much higher in MES-SA/Dx5, indicating the capability of the NPs to overcome P-glycoprotein resistance mechanisms. NP-encapsulated ICG showed slightly different subcellular localization, but similar fluorescence intensity when compared to free ICG, and retained the ability to generate heat for hyperthermia delivery.
CONCLUSION: The dual-agent-loaded system allowed for the simultaneous delivery of hyperthermia and chemotherapy, and this combinational treatment greatly improved cytotoxicity in MES-SA/Dx5 cells and to a lesser extent in SKOV-3 cells.
Authors:
Yuan Tang; Tingjun Lei; Romila Manchanda; Abhignyan Nagesetti; Alicia Fernandez-Fernandez; Supriya Srinivasan; Anthony J McGoron
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-06
Journal Detail:
Title:  Pharmaceutical research     Volume:  27     ISSN:  1573-904X     ISO Abbreviation:  Pharm. Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2242-53     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, Florida 33174, USA.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / administration & dosage*,  pharmacokinetics
Biological Transport
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Doxorubicin / administration & dosage*,  pharmacokinetics,  pharmacology
Drug Carriers / chemistry*
Drug Resistance, Neoplasm / drug effects
Female
Humans
Hyperthermia, Induced / methods
Lactic Acid / chemistry*
Microscopy, Electron, Scanning
Nanoparticles / chemistry*
Optical Phenomena
Organic Chemicals / administration & dosage*,  pharmacokinetics,  pharmacology
Particle Size
Polyglycolic Acid / chemistry*
Solubility
Surface Properties
Grant Support
ID/Acronym/Agency:
R25 GM061347/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Drug Carriers; 0/Organic Chemicals; 0/polylactic acid-polyglycolic acid copolymer; 23214-92-8/Doxorubicin; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 84133-51-7/iodocyanine green

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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