| Simultaneous delivery of chemotherapeutic and thermal-optical agents to cancer cells by a polymeric (PLGA) nanocarrier: an in vitro study. | |
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MedLine Citation:
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PMID: 20694526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To test the effectiveness of a dual-agent-loaded PLGA nanoparticulate drug delivery system containing doxorubicin (DOX) and indocyanine green (ICG) in a DOX-sensitive cell line and two resistant cell lines that have different resistance mechanisms. METHODS: The DOX-sensitive MES-SA uterine sarcoma cell line was used as a negative control. The two resistant cell lines were uterine sarcoma MES-SA/Dx5, which overexpresses the multidrug resistance exporter P-glycoprotein, and ovarian carcinoma SKOV-3, which is less sensitive to doxorubicin due to a p53 gene mutation. The cellular uptake, subcellular localization and cytotoxicity of the two agents when delivered via nanoparticles (NPs) were compared to their free-form administration. RESULTS: The cellular uptake and cytotoxicity of DOX delivered by NPs were comparable to the free form in MES-SA and SKOV-3, but much higher in MES-SA/Dx5, indicating the capability of the NPs to overcome P-glycoprotein resistance mechanisms. NP-encapsulated ICG showed slightly different subcellular localization, but similar fluorescence intensity when compared to free ICG, and retained the ability to generate heat for hyperthermia delivery. CONCLUSION: The dual-agent-loaded system allowed for the simultaneous delivery of hyperthermia and chemotherapy, and this combinational treatment greatly improved cytotoxicity in MES-SA/Dx5 cells and to a lesser extent in SKOV-3 cells. |
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Authors:
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Yuan Tang; Tingjun Lei; Romila Manchanda; Abhignyan Nagesetti; Alicia Fernandez-Fernandez; Supriya Srinivasan; Anthony J McGoron |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-06 |
Journal Detail:
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Title: Pharmaceutical research Volume: 27 ISSN: 1573-904X ISO Abbreviation: Pharm. Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2011-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: United States |
Other Details:
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Languages: eng Pagination: 2242-53 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, Florida 33174, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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administration & dosage*,
pharmacokinetics Biological Transport Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Doxorubicin / administration & dosage*, pharmacokinetics, pharmacology Drug Carriers / chemistry* Drug Resistance, Neoplasm / drug effects Female Humans Hyperthermia, Induced / methods Lactic Acid / chemistry* Microscopy, Electron, Scanning Nanoparticles / chemistry* Optical Phenomena Organic Chemicals / administration & dosage*, pharmacokinetics, pharmacology Particle Size Polyglycolic Acid / chemistry* Solubility Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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R25 GM061347/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Drug Carriers; 0/Organic Chemicals; 0/polylactic acid-polyglycolic acid copolymer; 23214-92-8/Doxorubicin; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 84133-51-7/iodocyanine green |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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