| Simulation of cell growth, substrate consumption and product formation with recombinant Escherichia coli. | |
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MedLine Citation:
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PMID: 1367369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The growth of recombinant Escherichia coli, consumption of different amino acids and glucose as well as fusion protein formation are simulated by a structured mathematical model and compared with measurements carried out in a stirred tank reactor under well-controlled conditions. The model parameters were identified based on the variation in cell concentration, particular amino acids, and glucose as well as intracellular products during cultivation. The dependence of the model parameters on cultivation time and culture conditions (temperature, medium composition, dissolved oxygen concentration) are discussed. |
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Authors:
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G Korte; U Rinas; H A Kracke-Helm; K Schügerl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 35 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-10-10 Completed Date: 1991-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 189-99 Citation Subset: B |
Affiliation:
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TUV Hannover, Abt. KTSS, Hannover-Kirchrode, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Bacterial Proteins / biosynthesis* Carbohydrate Metabolism Culture Media / metabolism* Escherichia coli / growth & development*, metabolism Gene Amplification Mathematics Models, Biological* Oxygen / metabolism Plasmids Temperature |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Proteins; 0/Culture Media; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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