Document Detail

Simulation of cell growth, substrate consumption and product formation with recombinant Escherichia coli.
MedLine Citation:
PMID:  1367369     Owner:  NLM     Status:  MEDLINE    
The growth of recombinant Escherichia coli, consumption of different amino acids and glucose as well as fusion protein formation are simulated by a structured mathematical model and compared with measurements carried out in a stirred tank reactor under well-controlled conditions. The model parameters were identified based on the variation in cell concentration, particular amino acids, and glucose as well as intracellular products during cultivation. The dependence of the model parameters on cultivation time and culture conditions (temperature, medium composition, dissolved oxygen concentration) are discussed.
G Korte; U Rinas; H A Kracke-Helm; K Schügerl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  35     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  189-99     Citation Subset:  B    
TUV Hannover, Abt. KTSS, Hannover-Kirchrode, Federal Republic of Germany.
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MeSH Terms
Amino Acids / metabolism
Bacterial Proteins / biosynthesis*
Carbohydrate Metabolism
Culture Media / metabolism*
Escherichia coli / growth & development*,  metabolism
Gene Amplification
Models, Biological*
Oxygen / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Culture Media; 7782-44-7/Oxygen

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