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Simulated nitrogen deposition affects community structure of arbuscular mycorrhizal fungi in northern hardwood forests.
MedLine Citation:
PMID:  21210962     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Our previous investigation found elevated nitrogen deposition caused declines in abundance of arbuscular mycorrhizal fungi (AMF) associated with forest trees, but little is known about how nitrogen affects the AMF community composition and structure within forest ecosystems. We hypothesized that N deposition would lead to significant changes in the AMF community structure. We studied the diversity and community structure of AMF in northern hardwood forests after more than 12 years of simulated nitrogen deposition. We performed molecular analyses on maple (Acer spp.) roots targeting the 18S rDNA region using the fungal-specific primers AM1 and NS31. PCR products were cloned and identified using restriction fragment length polymorphism (RFLP) and sequencing. N addition significantly altered the AMF community structure, and Glomus group A dominated the AMF community. Some Glomus operational taxonomic units (OTUs) responded negatively to N inputs, whereas other Glomus OTUs and an Acaulospora OTU responded positively to N inputs. The observed effect on community structure implies that AMF species associated with maples differ in their response to elevated nitrogen. Given that functional diversity exists among AMF species and that N deposition has been shown to select less beneficial fungi in some ecosystems, this change in community structure could have implications for the functioning of this type of ecosystem.
Authors:
Linda T A VAN Diepen; Erik A Lilleskov; Kurt S Pregitzer
Publication Detail:
Type:  Journal Article     Date:  2011-01-07
Journal Detail:
Title:  Molecular ecology     Volume:  20     ISSN:  1365-294X     ISO Abbreviation:  Mol. Ecol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  799-811     Citation Subset:  IM    
Copyright Information:
Published 2011. This article is a US Government work and is in the public domain in the USA.
Affiliation:
Ecosystem Science Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA USDA Forest Service, Northern Research Station, Houghton, MI 49931, USA College of Natural Resources, University of Idaho, Moscow, ID 83844, USA.
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