| Simplified synthesis and relaxometry of magnetoferritin for magnetic resonance imaging. | |
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MedLine Citation:
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PMID: 20677230 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Magnetoferritin nanoparticles have been developed as high-relaxivity, functional contrast agents for MRI. Several previous techniques have relied on unloading native ferritin and re-incorporation of iron into the core, often resulting in a polydisperse sample. Here, a simplified technique is developed using commercially available horse spleen apoferritin to create monodisperse magnetoferritin. Iron oxide atoms were incorporated into the protein core via a step-wise Fe(II)Chloride addition to the protein solution under low O(2) conditions; subsequent filtration steps allow for separation of completely filled and superparamagnetic magnetoferritin from the partially filled ferritin. This method yields a monodisperse and homogenous solution of spherical particles with magnetic properties that can be used for molecular magnetic resonance imaging. With a transverse per-iron and per-particle relaxivity of 78 mM(-1) sec(-1) and 404,045 mM(-1) sec(-1), respectively, it is possible to detect ∼ 10 nM nanoparticle concentrations in vivo. |
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Authors:
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Veronica Clavijo Jordan; Michael R Caplan; Kevin M Bennett |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 64 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 1260-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley-Liss, Inc. |
Affiliation:
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School of Biological and Health Systems Engineering, Ira A. Fulton School of Engineering, Arizona State University, Tempe, Arizona 85287-9709, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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