Document Detail


Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis.
MedLine Citation:
PMID:  22606689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400,000 theoretical plates.
Authors:
Oluwatosin O Dada; Bonnie J Huge; Norman J Dovichi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-05-18
Journal Detail:
Title:  The Analyst     Volume:  137     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-08     Completed Date:  2012-10-01     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  3099-101     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Capillary / methods*
Flow Cytometry
Limit of Detection
Spectrometry, Fluorescence / methods*
Grant Support
ID/Acronym/Agency:
R21 GM103462/GM/NIGMS NIH HHS; R21GM103462/GM/NIGMS NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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