| Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein. | |
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MedLine Citation:
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PMID: 23341608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A compendium of different types of abiotic chemical syntheses identifies a consensus set of 10 "prebiotic" α-amino acids. Before the emergence of biosynthetic pathways, this set is the most plausible resource for protein formation (i.e., proteogenesis) within the overall process of abiogenesis. An essential unsolved question regarding this prebiotic set is whether it defines a "foldable set"--that is, does it contain sufficient chemical information to permit cooperatively folding polypeptides? If so, what (if any) characteristic properties might such polypeptides exhibit? To investigate these questions, two "primitive" versions of an extant protein fold (the β-trefoil) were produced by top-down symmetric deconstruction, resulting in a reduced alphabet size of 12 or 13 amino acids and a percentage of prebiotic amino acids approaching 80%. These proteins show a substantial acidification of pI and require high salt concentrations for cooperative folding. The results suggest that the prebiotic amino acids do comprise a foldable set within the halophile environment. |
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Authors:
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Liam M Longo; Jihun Lee; Michael Blaber |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2013-01-22 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-02-06 Completed Date: 2013-04-09 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 2135-9 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, Florida State University, Tallahassee, FL 32306-4300, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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PDB/3Q7W; 3Q7X; 4D8H |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry* Biogenesis Biophysical Phenomena Calorimetry, Differential Scanning Crystallography, X-Ray Evolution, Molecular* Models, Molecular Mutagenesis Protein Folding Protein Stability Proteins / chemistry*, genetics Static Electricity |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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