Document Detail


Simplified flow cytometric method for fetal hemoglobin containing red blood cells.
MedLine Citation:
PMID:  11135294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Assay of fetal hemoglobin (HbF) and/or HbF containing red blood cells (F+ cells) is essential for monitoring sickle cell and thalassemic patients, especially during treatment with HbF stimulators. Some previous flow cytometric methods contain several washing steps. This simplified method contains no washing step and takes less than an hour to perform. The %F+ cells in five mixtures of fetal red blood cells with adult red blood cells were nonsignificantly different in the original and simplified procedure. The %F+ cells of 12 patients compared in these two procedures were also not significantly different. The intra- and interassay %CVs do not exceed 3% and 7% respectively. EDTA, citrate, or heparin is suitable as anticoagulant and the samples can be stored at 4 degrees C for up to 2 weeks. The %F+ cells and %HbF [by high-performance liquid chromatography (HPLC)] of 83 samples were highly significantly correlated regardless of diagnosis. In conclusion, this new simplified flow cytometric method for F+ cells is simple, convenient, rapid, reproducible, and could be applied for monitoring sickle cell and thalassemic patients as an alternative to HPLC, where this is unavailable. It can also be applied as a fetal cell assay in fetomaternal hemorrhage.
Authors:
Y Mundee; N C Bigelow; B H Davis; J B Porter
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Publication Detail:
Type:  Evaluation Studies; Technical Report    
Journal Detail:
Title:  Cytometry     Volume:  42     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-16     Completed Date:  2001-02-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  389-93     Citation Subset:  IM    
Affiliation:
Department of Haematology, University College London Medical School, London, UK. rmgvymu@ucl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Anemia, Sickle Cell / blood
Anticoagulants / chemistry
Erythrocytes / chemistry*
Fetal Hemoglobin / analysis*
Flow Cytometry / methods*
Humans
Reproducibility of Results
Thalassemia / blood
Chemical
Reg. No./Substance:
0/Anticoagulants; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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