| Simplified cell microelectrophoretic method applied to the macrophage electrophoretic mobility test for cancer diagnosis. | |
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MedLine Citation:
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PMID: 86499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Simplified agarose-coated capillary microelectrophoresis was adapted to the Macrophage Electrophoretic Mobility (MEM) test for cancer detection. Guinea pig alveolar macrophages gave superior reproducibility to peritoneal macrophages. As good or better reproducibility was obtained with cryopreserved, as with fresh macrophages. The electrophoretic mobilities of patients' lymphocytes themselves, after incubation with Encephalitogenic Factor (EF) showed a significant increase in electrophoretic mobility, while in control lymphocytes a decrease occurred. Thus, for the detection of the results of the interaction of EF on human lymphocytes, a direct lymphocyte electrophoretic mobility test may suffice, and guinea pig macrophages may no longer be required at all. |
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Authors:
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R M Fike; C J van Oss |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Immunological communications Volume: 8 ISSN: 0090-0877 ISO Abbreviation: Immunol. Commun. Publication Date: 1979 |
Date Detail:
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Created Date: 1979-07-28 Completed Date: 1979-07-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0353016 Medline TA: Immunol Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 173-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Electrophoresis, Cellulose Acetate Guinea Pigs Humans Lymphocytes / immunology Macrophages / immunology* Myelin Basic Proteins Neoplasms / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Myelin Basic Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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