Document Detail


Simplified cell culture method for the diagnosis of atypical primary ciliary dyskinesia.
MedLine Citation:
PMID:  19770168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The diagnosis of primary ciliary dyskinesia (PCD) can be challenging, and it may be particularly difficult to distinguish primary ciliary disease from the secondary changes after infections. OBJECTIVES: The purpose of the study was to evaluate if nasal epithelial cells, obtained with nasal brushing instead of a biopsy, could be used in a culture system for the diagnosis of PCD in difficult cases. Methods and MAIN RESULTS: Ciliary motion analysis (CMA) and transmission electron microscopy (TEM) were performed on 59 subjects with persistent or recurrent pneumonia. These investigations allowed the diagnosis of PCD in 13 (22%) patients while the defect of the cilia was considered secondary to infections in 37 (63%) subjects. In the remaining nine (15%) patients the diagnostic evaluation with CMA and TEM remained inconclusive. Ciliogenesis in culture allowed the diagnosis of PCD in four of these patients, it was indicative of a secondary defect in two subjects, and it was not helpful in the remaining three patients. CONCLUSIONS: Culture of cells obtained with brushing of the nasal turbinate is not a perfect test, nevertheless it may offer diagnostic help in doubtful cases of PCD.
Authors:
M Pifferi; F Montemurro; A M Cangiotti; V Ragazzo; M Di Cicco; B Vinci; G Vozzi; P Macchia; A L Boner
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-21
Journal Detail:
Title:  Thorax     Volume:  64     ISSN:  1468-3296     ISO Abbreviation:  Thorax     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-02     Completed Date:  2010-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417353     Medline TA:  Thorax     Country:  England    
Other Details:
Languages:  eng     Pagination:  1077-81     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Pisa, Via Roma 67, 56126 Pisa, Italy. m.pifferi@med.unipi.it
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cell Culture Techniques / methods
Child
Child, Preschool
Cilia / ultrastructure
Ciliary Motility Disorders / diagnosis
Diagnosis, Differential
Female
Humans
Infant
Kartagener Syndrome / diagnosis*
Male
Middle Aged
Nasal Mucosa / pathology
Specimen Handling / methods
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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