Document Detail


Simplified pulse reactor for real-time long-term in vitro testing of biological heart valves.
MedLine Citation:
PMID:  20204701     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Long-term function of biological heart valve prostheses (BHV) is limited by structural deterioration leading to failure with associated arterial hypertension. The objective of this work was development of an easy to handle real-time pulse reactor for evaluation of biological and tissue engineered heart valves under different pressures and long-term conditions. The pulse reactor was made of medical grade materials for placement in a 37 degrees C incubator. Heart valves were mounted in a housing disc moving horizontally in culture medium within a cylindrical culture reservoir. The microprocessor-controlled system was driven by pressure resulting in a cardiac-like cycle enabling competent opening and closing of the leaflets with adjustable pulse rates and pressures between 0.25 to 2 Hz and up to 180/80 mmHg, respectively. A custom-made imaging system with an integrated high-speed camera and image processing software allow calculation of effective orifice areas during cardiac cycle. This simple pulse reactor design allows reproducible generation of patient-like pressure conditions and data collection during long-term experiments.
Authors:
Martina Schleicher; Günther Sammler; Michael Schmauder; Olaf Fritze; Agnes J Huber; Katja Schenke-Layland; Günter Ditze; Ulrich A Stock
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-05
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  38     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-01     Completed Date:  2010-06-29     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1919-27     Citation Subset:  IM    
Affiliation:
Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tübingen, 72076 Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Heart Rate
Heart Valve Prosthesis / standards*
Heart Valves / physiopathology
Humans
Microcomputers
Physical Processes
Pressure
Time

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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