Document Detail


Simplified C-banding of mouse chromosomes.
MedLine Citation:
PMID:  2461274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse bone marrow chromosomes incorporated with bromodeoxyuridine (BrdU) were differentially Giemsa stained after exposure to incandescent light in order to study sister chromatid exchange. Over-exposure of the chromosomes to incandescent light often resulted in the complete loss of sister chromatid differentiation, and it frequently resulted in the C-band formation. C-banding was also prominent with chromosomes which were not labelled with BrdU. The method utilized a 300 W, 130 V, inside-frosted incandescent bulb coupled with phosphate buffering at pH 6.8 and an exposure period of 2 h.
Authors:
J R Edelman; Y J Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  54     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1988  
Date Detail:
Created Date:  1988-12-23     Completed Date:  1988-12-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  175-9     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, St John's University, Jamaica, New York 11439.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / analysis,  metabolism
Bone Marrow Cells
Bromodeoxyuridine / metabolism,  pharmacology
Chromosome Banding / methods*
Chromosomes / ultrastructure*
Light / diagnostic use
Metaphase
Mice
Sister Chromatid Exchange / drug effects
Staining and Labeling / methods
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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