Document Detail


Simple and specific electron-capture GLC assay for plasma and urine ephedrine concentrations following single doses.
MedLine Citation:
PMID:  430489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An electron-capture GLC procedure for determination of plasma ephedrine concentrations is described. The procedure is capable of determining 2 ng/ml of ephedrine and is adequate for following profiles after 25-mg single doses. Pentane extraction of the drug and the internal standard and formation of the N-pentafluorobenzoyl derivatives were followed by GLC. Plasma ephedrine concentrations following a 24-mg dose of ephedrine hydrochloride to a human volunteer are presented. Formation of N-trifluoroacetyl, N-pentafluoropropionyl, N-heptafluorobutryl, and N-pentafluorobenzoyl derivatives and their GLC-mass spectrometric identification are discussed together with comparative electron-capture sensitivities of these derivatives toward a nickel-63 detector. The detection of the N-pentafluorobenzoyl derivative of ephedrine is at least 100-fold greater in sensitivity than detection of the N-trifluoroacetyl derivative.
Authors:
K K Midha; J K Cooper; I J McGilveray
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical sciences     Volume:  68     ISSN:  0022-3549     ISO Abbreviation:  J Pharm Sci     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-06-29     Completed Date:  1979-06-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2985195R     Medline TA:  J Pharm Sci     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  557-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas / methods
Ephedrine / analysis*,  blood,  urine
Humans
Indicators and Reagents
Male
Methods
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 299-42-3/Ephedrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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