Document Detail


Simple sequence repeats in watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai).
MedLine Citation:
PMID:  9276932     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Simple sequence repeat length polymorphisms were utilized to examine genetic relatedness among accessions of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). A size-fractionated TaqI genomic library was screened for the occurrence of dimer and trimer simple sequence repeats (SSRs). A total of 96 (0.53%) SSR-bearing clones were identified and the inserts from 50 of these were sequenced. The dinucleotide repeats (CT)n and (GA)n accounted for 82% of the SSRs sequenced. PCR primer pairs flanking seven SSR loci were used to amplify SSRs from 32 morphologically variable watermelon genotypes from Africa, Europe, Asia, and Mexico and a single accession of Citrullus colocynthis from Chad. Cluster analysis of SSR length polymorphisms delineated 4 groups at the 25% level of genetic similarity. The largest group contained C. lanatus var. lanatus accessions. The second largest group contained only wild and cultivated "citron"-type or C. lanatus var. citroides accessions. The third group contained an accession tentatively identified as C. lanatus var. lanatus but which perhaps is a hybrid between C. lanatus var. lanatus and C. lanatus var. citroides. The fourth group consisted of a single accession identified as C. colocynthis. "Egusi"-type watermelons from Nigeria grouped with C. lanatus var. lanatus. The use of SSRs for watermelon germplasm characterization and genetic diversity studies is discussed.
Authors:
R L Jarret; L C Merrick; T Holms; J Evans; M K Aradhya
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  40     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-29     Completed Date:  1997-09-29     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  433-41     Citation Subset:  IM    
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Plant Genetic Resources, Griffin, GA 30223, USA.
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MeSH Terms
Descriptor/Qualifier:
DNA, Plant / genetics*
Plants, Edible / genetics*
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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