Document Detail


Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection.
MedLine Citation:
PMID:  11444702     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The USDA germplasm repositories help to preserve the genetic variability of important crop species by collecting and maintaining representative cultivars and related germplasm. Simple sequence repeat markers with high allelic diversity were used to type 41 grapevines from 40 accessions. All vines were either seedless table grape cultivars or cultivars with names similar to table grape cultivars. The proportion of shared alleles was selected as the most appropriate statistical measure of genetic distance for this population. In conjunction with morphological traits, known synonyms were confirmed and a previously unknown synonym was discovered. An alleged synonym in the literature was disproved by the DNA data. The data were consistent with known parentage, where such data were available. Two mislabeled vines in the USDA collection were identified. UPGMA grouped the cultivars loosely into three groups: a group of nine mostly Middle Eastern cultivars, a group of 22 accessions mostly from Russia and Afghanistan that were morphologically similar to 'Thompson Seedless', and a third very loose group of 11 accessions consisting mostly of eastern European wine grape cultivars. The limitations and usefulness of this type of analysis are discussed.
Authors:
G S Dangi; M L Mendum; B H Prins; M A Walker; C P Meredith; C J Simon
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  44     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-10     Completed Date:  2001-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  432-8     Citation Subset:  IM    
Affiliation:
US Department of Agriculture, National Clonal Germplasm Repository, University of California, Davis 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Cloning, Organism*
DNA, Plant / genetics*
Genetic Variation
Microsatellite Repeats / genetics*
Phylogeny*
Vitis / classification*,  cytology,  genetics*,  growth & development
Chemical
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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