| Simple, rapid, and sensitive liquid chromatography-fluorescence method for the quantification of tranexamic acid in blood. | |
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MedLine Citation:
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PMID: 17532325 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tranexamic acid (TA) is a synthetic antifibrinolytic agent that is being considered as a candidate adjuvant drug for site-specific pharmaco-laser therapy of port wine stains. For drug utility studies, a high-performance liquid chromatography (HPLC)-fluorescence method was developed for the quantification of TA in blood. Platelet-poor plasma was prepared, size-separated using 3kDa cut-off centrifuge filters, and derivatized with naphthalene-2-3-dicarboxaldehyde (NDA) and cyanide. The excess of NDA was quenched after 2 min by adding tryptophan. The derivatives were separated on a 2.1mm C18 column using an acetate buffer/acetonitrile gradient. Excellent separation from plasma background was obtained at pH 5.5. Quantification was carried out at 440/520 nm. The limit of detection was 0.5 microM and the mean+/-SD recovery from whole blood was 81.7+/-10.9%. Derivatized TA samples were stable for at least 36 h at 4 degrees C. The method was successfully applied to a heat-induced TA release study from thermosensitive liposomes. |
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Authors:
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José Fernando Huertas-Pérez; Michal Heger; Henk Dekker; Hans Krabbe; Jan Lankelma; Freek Ariese |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-06 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1157 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-02 Completed Date: 2007-11-07 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 142-50 Citation Subset: IM |
Affiliation:
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Department of Analytical Chemistry, University of Granada, Granada, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calibration Chromatography, High Pressure Liquid / methods* Reference Standards Sensitivity and Specificity Spectrometry, Fluorescence / methods* Tranexamic Acid / blood* |
| Chemical | |
Reg. No./Substance:
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1197-18-8/Tranexamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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