Document Detail


Simple, rapid, and sensitive liquid chromatography-fluorescence method for the quantification of tranexamic acid in blood.
MedLine Citation:
PMID:  17532325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tranexamic acid (TA) is a synthetic antifibrinolytic agent that is being considered as a candidate adjuvant drug for site-specific pharmaco-laser therapy of port wine stains. For drug utility studies, a high-performance liquid chromatography (HPLC)-fluorescence method was developed for the quantification of TA in blood. Platelet-poor plasma was prepared, size-separated using 3kDa cut-off centrifuge filters, and derivatized with naphthalene-2-3-dicarboxaldehyde (NDA) and cyanide. The excess of NDA was quenched after 2 min by adding tryptophan. The derivatives were separated on a 2.1mm C18 column using an acetate buffer/acetonitrile gradient. Excellent separation from plasma background was obtained at pH 5.5. Quantification was carried out at 440/520 nm. The limit of detection was 0.5 microM and the mean+/-SD recovery from whole blood was 81.7+/-10.9%. Derivatized TA samples were stable for at least 36 h at 4 degrees C. The method was successfully applied to a heat-induced TA release study from thermosensitive liposomes.
Authors:
José Fernando Huertas-Pérez; Michal Heger; Henk Dekker; Hans Krabbe; Jan Lankelma; Freek Ariese
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-06
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1157     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-02     Completed Date:  2007-11-07     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  142-50     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, University of Granada, Granada, Spain.
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MeSH Terms
Descriptor/Qualifier:
Calibration
Chromatography, High Pressure Liquid / methods*
Reference Standards
Sensitivity and Specificity
Spectrometry, Fluorescence / methods*
Tranexamic Acid / blood*
Chemical
Reg. No./Substance:
1197-18-8/Tranexamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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