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A SIMPLE ONE POT PURIFICATION OF BACTERIAL AMYLASE FROM FERMENTED BROTH BASED ON AFFINITY TOWARDS STARCH FUNCTIONALIZED MAGNETIC NANOPARTICLE.
MedLine Citation:
PMID:  24840788     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Surface functionalized adsorbant particles in combination with magnetic separation techniques have received considerable attention in recent years. Selective manipulation on such magnetic nanoparticles permits separation with high affinity in the presence of other suspended solids. Amylase is used extensively in food and allied industries. Purification of amylase from bacterial source is a matter of concern because most of the industrial need of amylase is met by microbial source. Here we report a simple, cost effective, one pot purification technique of bacterial amylase directly from fermented broth of Bacillus megaterium utilizing starch coated Superparamagnetic iron oxide nanoparticle (SPION). SPION was prepared by co-precipitation method and then functionalized by starch coating. The synthesized nanoparticles were characterized by TEM, SQUID, zeta potential, UV-vis and FTIR spectroscopy. The starch coated nanoparticles efficiently purified amylase from bacterial fermented broth with 93.22% recovery and 12.57 fold purification. SDS-PAGE revealed that the molecular weight of the purified amylase was 67 kDa and native gel showed the retention of amylase activity even after purification. Optimum pH and temperature of the purified amylase was 7 and 50 °C respectively and was stable over a range of 20 °C to 50 °C. Hence, an improved one pot bacterial amylase purification method was developed using starch coated SPION.
Authors:
Tanima Paul; Saptarshi Chatterjee; Arghya Bandyopadhyay; Dwiptirtha Chattopadhyay; Semanti Basu; Keka Sarkar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-19
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  -     ISSN:  1532-2297     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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