| Simple modular bioreactors for tissue engineering: a system for characterization of oxygen gradients, human mesenchymal stem cell differentiation, and prevascularization. | |
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MedLine Citation:
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PMID: 20528664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Large-scale tissue engineering is limited by nutrient perfusion and mass transport limitations, especially oxygen diffusion, which restrict construct development to smaller than clinically relevant dimensions and limit the ability for in vivo integration. The goal of this work was to develop a modular approach to tissue engineering, where scaffold and tissue size, transport issues, and surgical implantation in vivo are considered from the outset. Human mesenchymal stem cells (hMSCs) were used as the model cell type, as their differentiation has been studied for several different cell lineages and often with conflicting results. Changes in the expression profiles of hMSCs differentiated under varied oxygen tensions are presented, demonstrating tissue-specific oxygen requirements for both adipogenic (20% O₂) and chondrogenic (5% O₂) differentiation. Oxygen and nutrient transport were enhanced by developing a bioreactor system for perfusing hMSC-seeded collagen gels using porous silk tubes, resulting in enhanced oxygen transport and cell viability within the gels. These systems are simple to use and scaled for versatility, to allow for the systematic study of relationships between cell content, oxygen, and cell function. The data may be combined with oxygen transport modeling to derive minimally sized modular units for construction of clinically relevant tissue-engineered constructs, a generic strategy that may be employed for vascularized target tissues. |
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Authors:
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Michael Lovett; Danielle Rockwood; Amanda Baryshyan; David L Kaplan |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-13 |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-03-31 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 1565-73 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipogenesis
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physiology Bioreactors* Cell Culture Techniques / methods Cell Differentiation / physiology* Cells, Cultured Chondrogenesis / physiology Dose-Response Relationship, Drug Humans Mesenchymal Stem Cells / cytology, physiology* Neovascularization, Physiologic / physiology* Osmolar Concentration Oxygen / analysis*, pharmacology Tissue Engineering / instrumentation*, methods |
| Grant Support | |
ID/Acronym/Agency:
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EB002520/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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