Document Detail


Simple method to differentiate measles vaccine from wild-type strains using loop-mediated isothermal amplification (LAMP).
MedLine Citation:
PMID:  23336333     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Through increasing measles vaccine coverage, proportion of patients with modified measles has been increasing. They demonstrate low-grade fever with very mild eruptions similar to vaccine-related adverse events. Differentiation between the two pathogenic conditions is required to improve the quality of laboratory-based measles surveillance, In this report, vaccine-specific and wild-type specific primer sets were designed for the loop-mediated isothermal amplification (LAMP) in the N gene, and vaccine strains, C1, D3, D4, D5, D8, D9, G3, and H1 wild strains were examined. Three vaccine strains were efficiently amplified using a vaccine-specific primer set with an approximately 10-times higher sensitivity than wild-type primer. Modified measles was differentiated from vaccine-associated cases using this system, but with some limitations for the other genotypes.
Authors:
Tetsuo Nakayama; Akihito Sawada; Hideyuki Kubo; Atsushi Kaida; Toshimitsu Tanaka; Naoki Shigemoto; Katsuhiro Komase; Makoto Takeda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Kitasato Institute for Life Sciences, Laboratory of Virus Infection.
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