Document Detail

Simple method for separating the chlamydoconidia of Candida albicans from its mycelium.
MedLine Citation:
PMID:  3050528     Owner:  NLM     Status:  MEDLINE    
Different physical and chemical methods were used to detach the chlamydoconidia of Candida albicans from its mycelium. The action of concentrated H2SO4 acid for a 4-min period on cultures lysed both the mycelium and the outer but not the inner wall layer of the chlamydoconidia. The sulfuric acid procedure is recommended as the best method to obtain mycelium free chlamydoconidia because of its simplicity, rapidity and low cost.
V Vidotto; M Bruatto; M G Gallo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mycopathologia     Volume:  103     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-10-31     Completed Date:  1988-10-31     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  29-33     Citation Subset:  IM    
Istituto Malattie Infettive, Università degli Studi di Torino, Italy.
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MeSH Terms
Candida albicans / cytology,  isolation & purification*
Centrifugation, Density Gradient
Spores, Fungal / isolation & purification
Sulfuric Acids
Reg. No./Substance:
0/Sulfuric Acids; 7664-93-9/sulfuric acid

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