Document Detail

Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae.
MedLine Citation:
PMID:  892899     Owner:  NLM     Status:  MEDLINE    
Choleragenoid, a nontoxic aggregate of the B subunit of cholera toxin, has been purified from concentrated culture filtrates in a single step by ion-exchange chromatography on phosphocellulose or other cation-exchange resins. This procedure is far simpler than others currently used to isolate choleragenoid and yields a preparation essentially free from nucleic acid, lipopolysaccharide, toxin, and other proteins present in the crude culture filtrates. The purified choleragenoid retained the specific receptor-binding capacity of the toxin but exhibited no enterotoxic activity by either the ileal loop assay or the skin permeability assay. This purification methods may therefore be superior to others currently used for obtaining choleragenoid for immunization or other purposes.
J J Mekalanos; R J Collier; W R Romig
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  16     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-10-14     Completed Date:  1977-10-14     Revised Date:  2010-09-03    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  789-95     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Carbohydrates / analysis
Chromatography, Ion Exchange
Evaluation Studies as Topic
Skin Tests
Toxoids / analysis,  isolation & purification*,  toxicity
Vibrio cholerae / immunology*
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Toxoids

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