| Simple method for the determination of 5-aminosalicylic and N-acetyl-5-aminosalicylic acid in rectal tissue biopsies. | |
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MedLine Citation:
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PMID: 9824239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a sensitive high-performance liquid chromatographic method for the determination of 5-aminosalicylic acid and N-acetyl-5-aminosalicylic acid in rectal tissue biopsies. Samples were derivatised using propionic anhydride and proteins were precipitated with methanol. A Supelcosil ABZ column (150x4.6 mm I.D., 5 microm silica particles) was used with a mobile phase comprising 0.1 M acetic acid, acetonitrile and triethylamine (1600:114:6, v/v/v). Fluorescence detection was employed and detection limits were 0.2 ng/mg tissue at a signal-to-noise ratio of three (measured concentration: 5-aminosalicylic acid, 0.254 (0.228-0.286) ng/mg, C.V. 10.7%; N-acetyl-5-aminosalicylic acid, 0.18 (0.154-0.198) ng/mg, C.V 9.8%). This assay was validated for use with serum, urine and faecal samples for which it proved to be both precise and accurate (C.V.<10%, measured concentration within 10%). |
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Authors:
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F N Hussain; R A Ajjan; M Moustafa; J C Anderson; S A Riley |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 716 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1999-02-11 Completed Date: 1999-02-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 257-66 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Northern General Hospital, Sheffield, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminosalicylic Acids
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analysis*,
blood,
urine Anhydrides Biopsy* Chemical Precipitation Chromatography, High Pressure Liquid / methods* Colitis, Ulcerative / drug therapy Feces / chemistry Humans Indicators and Reagents Intestinal Mucosa / chemistry Irrigation Kinetics Mesalamine / analysis*, blood, urine Methanol Propionates Rectum / chemistry* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Aminosalicylic Acids; 0/Anhydrides; 0/Indicators and Reagents; 0/Propionates; 123-62-6/propionic anhydride; 51-59-2/N-acetyl-5-aminosalicylic acid; 67-56-1/Methanol; 89-57-6/Mesalamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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