Document Detail


Simple high-performance liquid chromatographic column-switching technique for the on-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolites in urine.
MedLine Citation:
PMID:  9518158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive, simple and rapid method without sample pretreatment is presented for the simultaneous determination of flunitrazepam and its main metabolites (norflunitrazepam, 7-amino- and 7-acetamidoflunitrazepam) in urine. The single-step procedure is based on a column-switching technique which uses an immobilized antibody in an extraction column following concentration on a precolumn and separation on an analytical column. UV detection was performed at 254 nm. The reusability of the antibody exceeds 88 runs and a complete analysis was performed in less than 40 min. The method shows coefficients of variation below 9.9% and rates of recovery greater than 92% tested at the level of 50 ng/ml urine. The limit of detection was below 2 ng/ml urine for the four compounds.
Authors:
I Deinl; L Angermaier; C Franzelius; G Machbert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  704     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-03-25     Completed Date:  1998-03-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  251-8     Citation Subset:  IM    
Affiliation:
Institute of Forensic Medicine, Erlangen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Anti-Anxiety Agents*
Centrifugation
Chromatography, High Pressure Liquid / methods*
Flunitrazepam / analogs & derivatives,  metabolism*,  urine*
Humans
Hydrogen-Ion Concentration
Immunosorbent Techniques*
Kinetics
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Anti-Anxiety Agents; 1622-62-4/Flunitrazepam; 34084-50-9/7-aminoflunitrazepam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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