| Simple determination of microbial protein in rumen liquor. | |
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MedLine Citation:
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PMID: 7153399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for microbial protein determination based on Lowry's assay was devised. Differential centrifugation was used to separate bacterial cells from rumen liquor. Proteins from cells were released by suspending in .25 N sodium hydroxide and heating in boiling water bath for 10 min. Protein was determined by the Folin phenol method. The method is simple, accurate, reproducible, sensitive, specific, brief, and can be performed with small amount of rumen liquor (5 ml). A large number of samples can be handled conveniently. |
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Authors:
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H P Makkar; O P Sharma; R K Dawra; S S Negi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 65 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-03-11 Completed Date: 1983-03-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2170-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / metabolism Bacterial Proteins / analysis* Cattle Gastric Juice / analysis Indicators and Reagents Male Rumen / analysis*, microbiology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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