Document Detail


Simple and accurate determination of global tau(R) in proteins using (13)C or (15)N relaxation data.
MedLine Citation:
PMID:  10698665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the study of protein dynamics by (13)C or (15)N relaxation measurements different models from the Lipari-Szabo formalism are used in order to determine the motion parameters. The global rotational correlation time tau(R) of the molecule must be estimated prior to the analysis. In this Communication, the authors propose a new approach in determining an accurate value for tau(R) in order to realize the best fit of R(2) for the whole sequence of the protein, regardless of the different type of motions atoms may experience. The method first determines the highly structured regions of the sequence. For each corresponding site, the Lipari-Szabo parameters are calculated for R(1) and NOE, using an arbitrary value for tau(R). The chi(2) for R(2), summed over the selected sites, shows a clear minimum, as a function of tau(R). This minimum is used to better estimate a proper value for tau(R).
Authors:
J Mispelter; N Izadi-Pruneyre; E Quiniou; E Adjadj
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of magnetic resonance (San Diego, Calif. : 1997)     Volume:  143     ISSN:  1090-7807     ISO Abbreviation:  J. Magn. Reson.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-10     Completed Date:  2000-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9707935     Medline TA:  J Magn Reson     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  229-32     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
U350 INSERM, Institut Curie, Laboratoires R. Latarjet, Orsay, F-91405, France. joel.mispelter@curie.u-psud.fr
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MeSH Terms
Descriptor/Qualifier:
Carbon Isotopes
Magnetic Resonance Spectroscopy / methods*
Nitrogen Isotopes
Proteins / chemistry*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Nitrogen Isotopes; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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