| Simple and accurate determination of global tau(R) in proteins using (13)C or (15)N relaxation data. | |
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MedLine Citation:
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PMID: 10698665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the study of protein dynamics by (13)C or (15)N relaxation measurements different models from the Lipari-Szabo formalism are used in order to determine the motion parameters. The global rotational correlation time tau(R) of the molecule must be estimated prior to the analysis. In this Communication, the authors propose a new approach in determining an accurate value for tau(R) in order to realize the best fit of R(2) for the whole sequence of the protein, regardless of the different type of motions atoms may experience. The method first determines the highly structured regions of the sequence. For each corresponding site, the Lipari-Szabo parameters are calculated for R(1) and NOE, using an arbitrary value for tau(R). The chi(2) for R(2), summed over the selected sites, shows a clear minimum, as a function of tau(R). This minimum is used to better estimate a proper value for tau(R). |
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Authors:
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J Mispelter; N Izadi-Pruneyre; E Quiniou; E Adjadj |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: 143 ISSN: 1090-7807 ISO Abbreviation: J. Magn. Reson. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-05-10 Completed Date: 2000-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 229-32 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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U350 INSERM, Institut Curie, Laboratoires R. Latarjet, Orsay, F-91405, France. joel.mispelter@curie.u-psud.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Isotopes Magnetic Resonance Spectroscopy / methods* Nitrogen Isotopes Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Nitrogen Isotopes; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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